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Appl Environ Microbiol. 1983 July; 46(1): 37-43

Amplification of the aroA gene from Escherichia coli results in tolerance to the herbicide glyphosate.

S G Rogers, L A Brand, S B Holder, E S Sharps and M J Brackin

ABSTRACT

The predominant cellular target of the herbicide glyphosate is thought to be the enzyme 5-enolpyruvylshikimate-3-phosphoric acid synthase (EPSP synthase). As a means of biologically testing this finding, we cloned a segment of DNA from Escherichia coli that encodes this enzyme. Clones carrying the gene for EPSP synthase were identified by genetic complementation. Cells that contain a multicopy plasmid carrying the EPSP synthase gene overproduce the enzyme 5- to 17-fold and exhibit at least an 8-fold increased tolerance to glyphosate. These experiments provide direct biological evidence that EPSP synthase is a major site of glyphosate action in E. coli and that, in an amplified form, it can serve as a selectable glyphosate resistance marker.

Appl Environ Microbiol. 1983 July; 46(1): 37-43




This article has been cited by other articles:

  • Dun, B.-Q., Wang, X.-J., Lu, W., Zhao, Z.-L., Hou, S.-N., Zhang, B.-M., Li, G.-Y., Evans, T. C. Jr., Xu, M.-Q., Lin, M. (2007). Reconstitution of Glyphosate Resistance from a Split 5-Enolpyruvyl Shikimate-3-Phosphate Synthase Gene in Escherichia coli and Transgenic Tobacco. Appl. Environ. Microbiol. 73: 7997-8000 [Abstract] [Full Text]  


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