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Appl Environ Microbiol. 1989 July; 55(7): 1852-1854
Copyright © 1989, American Society for Microbiology. All Rights Reserved.

Exopolysaccharide-Deficient Mutants of Rhizobium fredii HH303 Which Are Symbiotically Effective

Choong-Hyun Kim{dagger}, Raymond E. Tully and Donald L. Keister*

Nitrogen Fixation and Soybean Genetics Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Bldg. 011, HH-19, BARC-W, Beltsville, Maryland 20705

ABSTRACT

Nineteen Tn5-induced mutants of Rhizobium fredii HH303 defective in acidic exopolysaccharide synthesis were isolated by screening for lack of Calcofluor fluorescence. They were grouped by complementation analysis by using Rhizobium meliloti cosmids carrying exo genes. All of the 19 mutants were symbiotically effective or partially effective, indicating that the major bacterial acidic exopolysaccharide of this strain of R. fredii may not be required for symbiotic development in the soybean.

FOOTNOTES

* Corresponding author.

{dagger} Present address: University of California San Diego Medical Center, H811F, San Diego, CA 92103.

Appl Environ Microbiol. 1989 July; 55(7): 1852-1854
Copyright © 1989, American Society for Microbiology. All Rights Reserved.



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Copyright © 1989 by the American Society for Microbiology. All rights reserved.