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Appl Environ Microbiol. 1991 March; 57(3): 712-716
Copyright © 1991, American Society for Microbiology. All Rights Reserved.

Production of Alkaline Protease by a New Aspergillus flavus Isolate under Solid-Substrate Fermentation Conditions for Use as a Depilation Agent

S. Malathi* and R. Chakraborty

Department of Biochemistry, Central Leather Research Institute, Madras 600 020, India

ABSTRACT

The production of alkaline protease by an Aspergillus flavus strain isolated in our laboratory by solid-substrate fermentation for use as a depilation agent and the influence of various factors on enzyme production are reported. The optimum conditions for maximum production were a growth temperature of 32°C, 63% substrate moisture, and a growth period of 48 h. Enrichment with corn steep liquor or Casitone increased productivity. Scaling-up experiments indicated that flask-scale results could be reproduced at 1 and 30 kg of substrate. The enzyme preparation exhibited maximum activity at both pH 7.5 and pH 9.5. The use of this enzyme as a depilation agent was confirmed by experiments in a tannery.

FOOTNOTES

* Corresponding author.

Appl Environ Microbiol. 1991 March; 57(3): 712-716
Copyright © 1991, American Society for Microbiology. All Rights Reserved.



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Copyright © 1991 by the American Society for Microbiology. All rights reserved.