This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nicole, M Articles by Ouellette, G B Search for Related Content PubMed PubMed Citation Articles by Nicole, M Articles by Ouellette, G B Agricola Articles by Nicole, M Articles by Ouellette, G B

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1992 May; 58(5): 1727-1739

Immunocytochemical localization of laccase L1 in wood decayed by Rigidoporus lignosus.

M Nicole, H Chamberland, J P Geiger, N Lecours, J Valero, B Rio and G B Ouellette

Forestry Canada, Quebec Region, Sainte-Foy.

ABSTRACT

The cellular distribution of laccase L1 during degradation of wood chips by Rigidoporus lignosus, a tropical white rot fungus, was investigated by using anti-laccase L1 polyclonal antisera in conjunction with immunolabeling techniques. The enzyme was localized in the fungal cytoplasm and was associated with the plasmalemma and the fungal cell wall. An extracellular sheath, often observed around fungal cells, often contained laccase molecules. Diffusion of laccase within apparently unaltered wood was seldom observed. The enzyme penetrated all degraded cell walls, from the secondary wall toward the primary wall, including the middle lamella. Xylem cells showing advanced stages of decay were sometimes devoid of significant labeling. These data suggest that the initial attack on wood was not performed by laccase L1 of R. lignosus. Previous alteration of the lignocellulose complex may facilitate the movement of laccase within the wood cell walls. This immunogold study revealed that laccase localization during wood degradation seems limited not in space but in time.

---

Appl Environ Microbiol. 1992 May; 58(5): 1727-1739

This article has been cited by other articles:

• Barrasa, J. M., Gutiérrez, A., Escaso, V., Guillén, F., Martínez, M. J., Martínez, A. T. (1998). Electron and Fluorescence Microscopy of Extracellular Glucan and Aryl-Alcohol Oxidase during Wheat-Straw Degradation by Pleurotus eryngii. Appl. Environ. Microbiol. 64: 325-332 [Abstract] [Full Text]