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Appl Environ Microbiol. 1993 December; 59(12): 4189-4197
Copyright © 1993, American Society for Microbiology. All Rights Reserved.

Cloning and Characterization of Aerobactin Biosynthesis Genes of the Biological Control Agent Enterobacter cloacae

Joyce E. Loper*, Carol A. Ishimaru{dagger}, Susan R. Carnegie and Apichart Vanavichit{ddagger}

Horticultural Crops Research Laboratory, Agricultural Research Service, United States Department of Agriculture, 3420 N.W. Orchard Avenue, and Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon 97330

ABSTRACT

Five strains of Enterobacter cloacae that are biological control agents of Pythium damping-off diseases produced the hydroxamate siderophore aerobactin under iron-limiting conditions. Genes determining aerobactin biosynthesis of the biocontrol strain E. cloacae EcCT-501 were localized to a 12.3-kb region, which conferred aerobactin production to Escherichia coli DH5{alpha}. The aerobactin biosynthesis genes of E. cloacae hybridized to those of the pColV-K30 plasmid of E. coli, but restriction patterns of the aerobactin regions of pColV-K30 and E. cloacae differed. A derivative strain with a deletion in the aerobactin biosynthesis locus was as effective as strain EcCT-501 in biological control of Pythium damping-off of cucumber. Thus, aerobactin production did not contribute significantly to the biological control activity of EcCT-501 under the conditions of this study.

FOOTNOTES

* Corresponding author.

{dagger} Present address: Department of Plant Pathology and Weed Science, Colorado State University, Fort Collins, CO 80523.

{ddagger} Present address: Kasetsart University, Bangkhen, Bangkok 10903, Thailand.

Appl Environ Microbiol. 1993 December; 59(12): 4189-4197
Copyright © 1993, American Society for Microbiology. All Rights Reserved.



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