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Appl Environ Microbiol. 1993 December; 59(12): 4253-4260
Copyright © 1993, American Society for Microbiology. All Rights Reserved.

Analysis of Fungal Pellets by UV-Visible Spectrum Diffuse Reflectance Spectroscopy

Domen Lestan{dagger},*, Helena Podgornik and Anton Perdih

Department of Chemistry and Chemical Technology, University of Ljubljana, P.O. Box 537, SLO-61001 Ljubljana, Slovenia

ABSTRACT

The application of the UV-visible spectrum diffuse reflectance spectroscopy for the determination of intracellular pH in vivo, for determination of cytochrome content, and for the noninvasive in vivo detection of the redox state of fungal mitochondrial cytochromes in filamentous fungi is introduced. The time course of the intracellular pH values, mitochondrial cytochromes, and CO-binding pigments content and the correlations between the actual redox state of cytochrome aa3 and saturation of growth medium with oxygen in pellets of the basidiomycete Phanerochaete chrysosporium were determined. As the test microorganism, the yeast Saccharomyces cerevisiae was used. UV-visible spectrum diffuse reflectance spectroscopy proved to be a promising method for the quick and simple analysis of light-impermeable biological structures for which the classical transmittance spectrophotometric methods are difficult to implement.

FOOTNOTES

* Corresponding author.

{dagger} Present address: Teslova ulica 6, 61000 Ljublijana, Slovenia.

Appl Environ Microbiol. 1993 December; 59(12): 4253-4260
Copyright © 1993, American Society for Microbiology. All Rights Reserved.



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