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Appl Environ Microbiol. 1993 December; 59(12): 4317-4322
Copyright © 1993, American Society for Microbiology. All Rights Reserved.

Glutamine Involvement in Nitrogen Control of Gibberellic Acid Production in Gibberella fujikuroi

Gastón A. Muñoz and Eduardo Agosin*

Department of Chemical Engineering, Faculty of Engineering, Catholic University of Chile, P.O. Box 6177, Santiago, Chile

ABSTRACT

When the fungus Gibberella fujikuroi ATCC 12616 was grown in fermentor cultures, both intracellular kaurene biosynthetic activities and extracellular GA3 accumulation reached high levels when exogenous nitrogen was depleted in the culture. Similar patterns were exhibited by several nonrelated enzymatic activities, such as formamidase and urease, suggesting that all are subject to nitrogen regulation. The behavior of the enzymes involved in nitrogen assimilation (glutamine synthetase, glutamate dehydrogenase, and glutamate synthase) during fungal growth in different nitrogen sources suggests that glutamine is the final product of nitrogen assimilation in G. fujikuroi. When ammonium or glutamine was added to hormone-producing cultures, extracellular GA3 did not accumulate. However, when the conversion of ammonium into glutamine was inhibited by L-methionine-DL-sulfoximine, only glutamine maintained this effect. These results suggest that glutamine may well be the metabolite effector in nitrogen repression of GA3 synthesis, as well as in other nonrelated enzymatic activities in G. fujikuroi.

FOOTNOTES

* Corresponding author.

Appl Environ Microbiol. 1993 December; 59(12): 4317-4322
Copyright © 1993, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Tudzynski, B., Rojas, M. C., Gaskin, P., Hedden, P. (2002). The Gibberellin 20-Oxidase of Gibberella fujikuroi Is a Multifunctional Monooxygenase. J. Biol. Chem. 277: 21246-21253 [Abstract] [Full Text]  
  • Fraser, J. A., Davis, M. A., Hynes, M. J. (2001). The Formamidase Gene of Aspergillus nidulans: Regulation by Nitrogen Metabolite Repression and Transcriptional Interference by an Overlapping Upstream Gene. Genetics 157: 119-131 [Abstract] [Full Text]  


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