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Appl Environ Microbiol. 1993 December; 59(12): 4342-4346
Copyright © 1993, American Society for Microbiology. All Rights Reserved.

Isolation and Characterization of a Novel Atrazine Metabolite Produced by the Fungus Pleurotus pulmonarius, 2-Chloro-4-Ethylamino-6-(1-Hydroxyisopropyl)Amino-1,3,5-Triazine

Segula Masaphy*, Dan Levanon, Jacob Vaya and Yigal Henis

1 Migal Technological Center, Kiryat Shmona 10200, Institute of Soil and Water, Volcani Center, Bet Dagan, 2 and Otto Warburg Center for Biotechnology in Agriculture, The Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot 76100, 3 Israel

ABSTRACT

The white rot fungus Pleurotus pulmonarius exhibited metabolism of atrazine (2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine) in liquid culture, producing the dealkylated metabolites desethylatrazine, desisopropylatrazine, and desethyl-desisopropylatrazine. A fourth, unknown metabolite was also produced. It was isolated and was identified as 2-chloro-4-ethylamino-6-(1-hydroxyisopropyl)amino-1,3,5-triazine by gas chromatography-mass spectrometry, Fourier transformed infrared spectroscopy, and 1H nuclear magnetic resonance analysis. The structure of this metabolite was confirmed by chemical synthesis of the compound and comparison with the fungally produced metabolite.

FOOTNOTES

* Corresponding author.

Appl Environ Microbiol. 1993 December; 59(12): 4342-4346
Copyright © 1993, American Society for Microbiology. All Rights Reserved.



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