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Appl Environ Microbiol. 1993 December; 59(12): 4363-4366
Copyright © 1993, American Society for Microbiology. All Rights Reserved.

Minimal Requirements for Exponential Growth of Lactococcus lactis

Peter Ruhdal Jensen and Karin Hammer*

2 Department of Microbiology, Technical University of Denmark, DK-2800 Lyngby, Denmark, 2 and Division of Molecular Biology, Netherlands Cancer Institute H5, NL-1066-CX, Amsterdam, The Netherlands

ABSTRACT

A minimal growth medium containing glucose, acetate, vitamins, and eight amino acids allowed for growth of Lactococcus lactis subsp. lactis, with a specific growth rate in batch culture of µ = 0.3 h-1. With 19 amino acids added, the growth rate increased to µ = 0.7 h-1 and the exponential growth phase proceeded until high cell concentrations were reached. We show that morpholinepropanesulfonic acid (MOPS) is a suitable buffer for L. lactis and may be applied in high concentrations.

FOOTNOTES

* Corresponding author.

Appl Environ Microbiol. 1993 December; 59(12): 4363-4366
Copyright © 1993, American Society for Microbiology. All Rights Reserved.



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