This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Weusthuis, R A Articles by van Dijken, J P Search for Related Content PubMed PubMed Citation Articles by Weusthuis, R A Articles by van Dijken, J P Agricola Articles by Weusthuis, R A Articles by van Dijken, J P

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1993 September; 59(9): 3102-3109

Energetics and kinetics of maltose transport in Saccharomyces cerevisiae: a continuous culture study.

R A Weusthuis, H Adams, W A Scheffers and J P van Dijken

Department of Microbiology and Enzymology, Kluyver Laboratory of Biotechnology, Delft University of Technology, The Netherlands.

ABSTRACT

In Saccharomyces cerevisiae, maltose is transported by a proton symport mechanism, whereas glucose transport occurs via facilitated diffusion. The energy requirement for maltose transport was evaluated with a metabolic model based on an experimental value of YATP for growth on glucose and an ATP requirement for maltose transport of 1 mol.mol-1. The predictions of the model were verified experimentally with anaerobic, sugar-limited chemostat cultures growing on a range of maltose-glucose mixtures at a fixed dilution rate of 0.1 h-1. The biomass yield (grams of cells.gram of sugar-1) decreased linearly with increasing amounts of maltose in the mixture. The yield was 25% lower during growth on maltose than during that on glucose, in agreement with the model predictions. During sugar-limited growth, the residual concentrations of maltose and glucose in the culture increased in proportion to their relative concentrations in the medium feed. From the residual maltose concentration, the in situ rates of maltose consumption by cultures, and the Km of the maltose carrier for maltose, it was calculated that the amount of this carrier was proportional to the in situ maltose consumption rate. This was also found for the amount of intracellular maltose. These two maltose-specific enzymes therefore exert high control over the maltose flux in S. cerevisiae in anaerobic, sugar-limited, steady-state cultures.

---

Appl Environ Microbiol. 1993 September; 59(9): 3102-3109

This article has been cited by other articles:

• Guimaraes, P. M. R., Multanen, J.-P., Domingues, L., Teixeira, J. A., Londesborough, J. (2008). Stimulation of Zero-trans Rates of Lactose and Maltose Uptake into Yeasts by Preincubation with Hexose To Increase the Adenylate Energy Charge. Appl. Environ. Microbiol. 74: 3076-3084 [Abstract] [Full Text]  
• Jansen, M. L. A., Daran-Lapujade, P., de Winde, J. H., Piper, M. D. W., Pronk, J. T. (2004). Prolonged Maltose-Limited Cultivation of Saccharomyces cerevisiae Selects for Cells with Improved Maltose Affinity and Hypersensitivity. Appl. Environ. Microbiol. 70: 1956-1963 [Abstract] [Full Text]  
• Jansen, M. L. A., De Winde, J. H., Pronk, J. T. (2002). Hxt-Carrier-Mediated Glucose Efflux upon Exposure of Saccharomyces cerevisiae to Excess Maltose. Appl. Environ. Microbiol. 68: 4259-4265 [Abstract] [Full Text]  
• van Dijken, J. P., van Tuijl, A., Luttik, M. A. H., Middelhoven, W. J., Pronk, J. T. (2002). Novel Pathway for Alcoholic Fermentation of {delta}-Gluconolactone in the Yeast Saccharomyces bulderi. J. Bacteriol. 184: 672-678 [Abstract] [Full Text]  
• Overkamp, K. M., Bakker, B. M., Kötter, P., van Tuijl, A., de Vries, S., van Dijken, J. P., Pronk, J. T. (2000). In Vivo Analysis of the Mechanisms for Oxidation of Cytosolic NADH by Saccharomyces cerevisiae Mitochondria. J. Bacteriol. 182: 2823-2830 [Abstract] [Full Text]  
• Kovarova-Kovar, K., Egli, T. (1998). Growth Kinetics of Suspended Microbial Cells: From Single-Substrate-Controlled Growth to Mixed-Substrate Kinetics. Microbiol. Mol. Biol. Rev. 62: 646-666 [Abstract] [Full Text]