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Appl Environ Microbiol. 1994 June; 60(6): 2164-2167

Bacillus DNA in fossil bees: an ancient symbiosis?

R J Cano, M K Borucki, M Higby-Schweitzer, H N Poinar, G O Poinar Jr and K J Pollard

Biological Sciences Department, California Polytechnic State University, San Luis Obispo 93407.

ABSTRACT

We report here the isolation of DNA from abdominal tissue of four extinct stingless bees (Proplebeia dominicana) in Dominican amber, PCR amplification of a 546-bp fragment of the 16S rRNA gene from Bacillus spp., and their corresponding nucleotide sequences. These sequences were used in basic local alignment search tool searches of nonredundant nucleic acid data bases, and the highest scores were obtained with 16S rRNA sequences from Bacillus spp. Phylogenetic inference analysis by the maximum-likelihood method revealed close phylogenetic relationships of the four presumed ancient Bacillus sequences with Bacillus pumilus, B. firmus, B. subtilis, and B. circulans. These four extant Bacillus spp. are commonly isolated from abdominal tissue of stingless bees. The close phylogenetic association of the extracted DNA sequences with these bee colonizers suggests that a similar bee-Bacillus association existed in the extinct species P. dominicana.

Appl Environ Microbiol. 1994 June; 60(6): 2164-2167




This article has been cited by other articles:

  • Gatson, J. W., Benz, B. F., Chandrasekaran, C., Satomi, M., Venkateswaran, K., Hart, M. E. (2006). Bacillus tequilensis sp. nov., isolated from a 2000-year-old Mexican shaft-tomb, is closely related to Bacillus subtilis.. Int. J. Syst. Evol. Microbiol. 56: 1475-1484 [Abstract] [Full Text]  
  • (2005). LEPTOTRICHITES RESINATUS NEW GENUS AND SPECIES: A FOSSIL SHEATHED BACTERIUM IN ALPINE CRETACEOUS AMBER. Journal of Paleontology 79: 175-184  
  • Cano, R., Borucki, M. (1995). Revival and identification of bacterial spores in 25- to 40-million-year-old Dominican amber. Science 268: 1060-1064 [Abstract]  


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