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Appl Environ Microbiol. 1994 August; 60(8): 2944-2948
Copyright © 1994, American Society for Microbiology. All Rights Reserved.

Culturability and Expression of Outer Membrane Proteins during Carbon, Nitrogen, or Phosphorus Starvation of Pseudomonas fluorescens DF57 and Pseudomonas putida DF14

Lene Kragelund^* and Ole Nybroe

¹ Microbiology Section, Department of Ecology and Molecular Biology, The Royal Veterinary and Agricultural University, DK-1958 Frederiksberg, Denmark

ABSTRACT

Changes in culturability and outer membrane protein profiles were investigated in Pseudomonas fluorescens DF57 and Pseudomonas putida DF14 during starvation for carbon, nitrogen, and phosphorus. P. fluorescens DF57 remained fully culturable for 4 days in all starvation regimes. The cell mass increased during starvation for nitrogen and phosphorus, indicating the accumulation of storage compounds, whereas it decreased slightly in carbon-starved cells. P. putida DF14 lost culturability during phosphorus starvation, and the mass of phosphate-starved cells did not increase. Analysis of additional P. fluorescens and P. putida strains, however, showed that the ability to preserve culturability during phosphorus starvation was not species but strain dependent. In DF57, an outer membrane protein of 55 kDa appeared during starvation for phosphorus, while another protein of 63 kDa was seen during all starvation conditions. DF14 induced two outer membrane proteins of 28 and 29 kDa during starvation for carbon and nitrogen, but no phosphorus-specific starvation protein could be detected. Therefore, starvation-induced outer membrane proteins do not seem to be conserved among the fluorescent pseudomonads and a unique starvation response might be found in individual strains.

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FOOTNOTES

^* Corresponding author. Mailing address: Microbiology Section, Department of Ecology and Molecular Biology, The Royal Veterinary and Agricultural University, Rolighedsvej 21, DK-1958 Frederiksberg, Denmark. Phone: 45 3528 2630. Fax: 45 3528 2624.

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Appl Environ Microbiol. 1994 August; 60(8): 2944-2948
Copyright © 1994, American Society for Microbiology. All Rights Reserved.

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