This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kaneko, S
Right arrow Articles by Kusakabe, I
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kaneko, S
Right arrow Articles by Kusakabe, I
Agricola
Right arrow Articles by Kaneko, S
Right arrow Articles by Kusakabe, I

 Previous Article  |  Next Article 

Appl Environ Microbiol. 1994 September; 60(9): 3425-3428

Purification and some properties of alpha-L-arabinofuranosidase from Bacillus subtilis 3-6.

S Kaneko, M Sano and I Kusakabe

Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.

ABSTRACT

alpha-L-Arabinofuranosidase (EC 3.2.1.55) was purified from culture supernatant of Bacillus subtilis 3-6. The enzyme had a molecular weight of 61,000 and displayed maximum activity at pH 7.0 and 60 degrees C. It released arabinose from O-alpha-L-arabinofuranosyl-(1-->3)-O-beta-D-xylopyranosyl-(1-->4)-D-x ylopyranos e (A1X2), O-beta-D-xylopyranosyl-(1-->4)-[O-alpha-L-arabinofuranosyl-(1-->3)]- O-beta-D-xylopyranosyl-(1-->4)-D-xylopyranose (A1X3), and arabinan, but not from O-beta-D-xylopyranosyl-(1-->2)-O-alpha-L- arabinofuranosyl-(1-->3)-O-beta-D-xylopyranosyl-(1-->4)-O-beta-D-xylopyr anosyl- (1-->4)-D-xylopyranose (A1X4), arabinoxylan, gum arabic, or arabinogalactan.

Appl Environ Microbiol. 1994 September; 60(9): 3425-3428




This article has been cited by other articles:

  • Inacio, J. M., Correia, I. L., de Sa-Nogueira, I. (2008). Two distinct arabinofuranosidases contribute to arabino-oligosaccharide degradation in Bacillus subtilis. Microbiology 154: 2719-2729 [Abstract] [Full Text]  
  • Inacio, J. M., de Sa-Nogueira, I. (2008). Characterization of abn2 (yxiA), Encoding a Bacillus subtilis GH43 Arabinanase, Abn2, and Its Role in Arabino-Polysaccharide Degradation. J. Bacteriol. 190: 4272-4280 [Abstract] [Full Text]  
  • Inacio, J. M., de Sa-Nogueira, I. (2007). trans-Acting Factors and cis Elements Involved in Glucose Repression of Arabinan Degradation in Bacillus subtilis. J. Bacteriol. 189: 8371-8376 [Abstract] [Full Text]  
  • Raposo, M. P., Inacio, J. M., Mota, L. J., de Sa-Nogueira, I. (2004). Transcriptional Regulation of Genes Encoding Arabinan-Degrading Enzymes in Bacillus subtilis. J. Bacteriol. 186: 1287-1296 [Abstract] [Full Text]  
  • Debeche, T., Cummings, N., Connerton, I., Debeire, P., O'Donohue, M. J. (2000). Genetic and Biochemical Characterization of a Highly Thermostable alpha -L-Arabinofuranosidase from Thermobacillus xylanilyticus. Appl. Environ. Microbiol. 66: 1734-1736 [Abstract] [Full Text]  
  • Kaneko, S., Arimoto, M., Ohba, M., Kobayashi, H., Ishii, T., Kusakabe, I. (1998). Purification and Substrate Specificities of Two alpha -L-Arabinofuranosidases from Aspergillus awamori IFO 4033. Appl. Environ. Microbiol. 64: 4021-4027 [Abstract] [Full Text]  
  • Saha, B. C., Bothast, R. J. (1998). Purification and Characterization of a Novel Thermostable alpha -L-Arabinofuranosidase from a Color-Variant Strain of Aureobasidium pullulans. Appl. Environ. Microbiol. 64: 216-220 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»