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Appl. Environ. Microbiol., 01 1995, 245-251, Vol 61, No. 1
Copyright © 1995, American Society for Microbiology

Application of ribotyping for differentiating Vibrio cholerae non-O1 isolated from shrimp farms in Thailand

A Dalsgaard, P Echeverria, JL Larsen, R Siebeling, O Serichantalergs and HH Huss
Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.

A collection of 143 Vibrio cholerae non-O1 strains isolated from shrimp farms in Thailand were characterized and grouped by ribotyping. Sixty- four ribotypes were distinguished following digestion of chromosomal DNA with the restriction enzyme BglI, and the reproducibility of the method was 100%. There was no correlation between specific ribotype distributions and the locations of the shrimp farms. Ribotype similarity was examined by cluster analysis, and two main groups with 10 and 54 ribotypes, respectively, were found. Correlation between ribotype and O-antigen expression was shown to exist among those isolates tested. Ribotyping appears to be a suitable method for differentiating environmental V. cholerae non-O1 strains, and comparison of ribotype patterns showed a high degree of genetic divergence within V. cholerae non-O1.

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