This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Conner, D. E.
Right arrow Articles by Kotrola, J. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Conner, D. E.
Right arrow Articles by Kotrola, J. S.
Agricola
Right arrow Articles by Conner, D. E.
Right arrow Articles by Kotrola, J. S.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., Jan 1995, 382-385, Vol 61, No. 1
Copyright © 1995, American Society for Microbiology

Growth and survival of Escherichia coli O157:H7 under acidic conditions

DE Conner and JS Kotrola
Department of Poultry Science, Auburn University, Alabama 36849-5416.

The effect of pH reduction with acetic (pH 5.2), citric (pH 4.0), lactic (pH 4.7), malic (pH 4.0), mandelic (pH 5.0), or tartaric (pH 4.1) acid on growth and survival of Escherichia coli O157:H7 in tryptic soy broth with 0.6% yeast extract held at 25, 10, or 4 degrees C for 56 days was determined. Triplicate flasks were prepared for each acid treatment at each temperature. At 25 degrees C, populations increased 2 to 4 log10 CFU/ml in all treatments except that with mandelic acid, whereas no growth occurred at 10 or 4 degrees C in any treatments except the control. However, at all sampling times, higher (P < 0.05) populations were recovered from treatments held at 4 degrees C than from those held at 10 degrees C. At 10 degrees C, E. coli O157:H7 was inactivated at higher rates in citric, malic, and mandelic acid treatments than in the other treatments. At the pH values tested, the presence of the organic acids enhanced survival of the pathogen at 4 degrees C compared with the unacidified control. E. coli O157:H7 has the ability to survive in acidic conditions (pH, > or = 4.0) for up to 56 days, but survival is affected by type of acidulant and temperature.


This article has been cited by other articles:

  • Bjornsdottir, K., Breidt, F. Jr., McFeeters, R. F. (2006). Protective Effects of Organic Acids on Survival of Escherichia coli O157:H7 in Acidic Environments. Appl. Environ. Microbiol. 72: 660-664 [Abstract] [Full Text]  
  • Samelis, J., Sofos, J. N., Kendall, P. A., Smith, G. C. (2005). Survival or Growth of Escherichia coli O157:H7 in a Model System of Fresh Meat Decontamination Runoff Waste Fluids and Its Resistance to Subsequent Lactic Acid Stress. Appl. Environ. Microbiol. 71: 6228-6234 [Abstract] [Full Text]  
  • Stewart, N., Feng, J., Liu, X., Chaudhuri, D., Foster, J. W., Drolet, M., Tse-Dinh, Y.-C. (2005). Loss of topoisomerase I function affects the RpoS-dependent and GAD systems of acid resistance in Escherichia coli. Microbiology 151: 2783-2791 [Abstract] [Full Text]  
  • Asahara, T., Shimizu, K., Nomoto, K., Hamabata, T., Ozawa, A., Takeda, Y. (2004). Probiotic Bifidobacteria Protect Mice from Lethal Infection with Shiga Toxin-Producing Escherichia coli O157:H7. Infect. Immun. 72: 2240-2247 [Abstract] [Full Text]  
  • Samartzidou, H., Mehrazin, M., Xu, Z., Benedik, M. J., Delcour, A. H. (2003). Cadaverine Inhibition of Porin Plays a Role in Cell Survival at Acidic pH. J. Bacteriol. 185: 13-19 [Abstract] [Full Text]  
  • Samelis, J., Sofos, J. N. (2002). Role of Glucose in Enhancing the Temperature-Dependent Growth Inhibition of Escherichia coli O157:H7 ATCC 43895 by a Pseudomonas sp.. Appl. Environ. Microbiol. 68: 2600-2604 [Abstract] [Full Text]  
  • Heimer, S. R., Welch, R. A., Perna, N. T., Posfai, G., Evans, P. S., Kaper, J. B., Blattner, F. R., Mobley, H. L. T. (2002). Urease of Enterohemorrhagic Escherichia coli: Evidence for Regulation by Fur and a trans-Acting Factor. Infect. Immun. 70: 1027-1031 [Abstract] [Full Text]  
  • Reinders, R. D., Biesterveld, S., Bijker, P. G. H. (2001). Survival of Escherichia coli O157:H7 ATCC 43895 in a Model Apple Juice Medium with Different Concentrations of Proline and Caffeic Acid. Appl. Environ. Microbiol. 67: 2863-2866 [Abstract] [Full Text]  
  • Berry, E. D., Cutter, C. N. (2000). Effects of Acid Adaptation of Escherichia coli O157:H7 on Efficacy of Acetic Acid Spray Washes To Decontaminate Beef Carcass Tissue. Appl. Environ. Microbiol. 66: 1493-1498 [Abstract] [Full Text]  
  • Castanie-Cornet, M.-P., Penfound, T. A., Smith, D., Elliott, J. F., Foster, J. W. (1999). Control of Acid Resistance in Escherichia coli. J. Bacteriol. 181: 3525-3535 [Abstract] [Full Text]  
  • Jordan, S. L., Glover, J., Malcolm, L., Thomson-Carter, F. M., Booth, I. R., Park, S. F. (1999). Augmentation of Killing of Escherichia coli O157 by Combinations of Lactate, Ethanol, and Low-pH Conditions. Appl. Environ. Microbiol. 65: 1308-1311 [Abstract] [Full Text]  
  • Cody, S. H., Glynn, M. K., Farrar, J. A., Cairns, K. L., Griffin, P. M., Kobayashi, J., Fyfe, M., Hoffman, R., King, A. S., Lewis, J. H., Swaminathan, B., Bryant, R. G., Vugia, D. J. (1999). An Outbreak of Escherichia coli O157:H7 Infection from Unpasteurized Commercial Apple Juice. ANN INTERN MED 130: 202-209 [Abstract] [Full Text]  
  • Gyles, C., Johnson, R., Gao, A., Ziebell, K., Pierard, D., Aleksic, S., Boerlin, P. (1998). Association of Enterohemorrhagic Escherichia coli Hemolysin with Serotypes of Shiga-Like-Toxin-Producing Escherichia coli of Human and Bovine Origins. Appl. Environ. Microbiol. 64: 4134-4141 [Abstract] [Full Text]  
  • Garcia-Graells, C., Hauben, K. J. A., Michiels, C. W. (1998). High-Pressure Inactivation and Sublethal Injury of Pressure-Resistant Escherichia coli Mutants in Fruit Juices. Appl. Environ. Microbiol. 64: 1566-1568 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»