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Appl. Environ. Microbiol., 01 1995, 397-398, Vol 61, No. 1
Copyright © 1995, American Society for Microbiology

Inhibition enzyme-linked immunosorbent assay for detection of Pseudomonas fluorescens on meat surfaces

PV Eriksson, GN di Paola, MF Pasetti and MA Manghi
Instituto de Estudios de la Inmunidad Humoral, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Argentina.

An inhibition enzyme-linked immunosorbent assay was developed for Pseudomonas fluorescens enumeration of meat surfaces. The assay detected contamination levels as low as 3 x 10(5) bacteria per ml and could be completed within 4 h. It could be used as a framework for a test system for quantifying P. fluorescens spoilage in meat products.