This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Neilan, B. A.
Right arrow Articles by Goodman, A. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Neilan, B. A.
Right arrow Articles by Goodman, A. E.
Agricola
Right arrow Articles by Neilan, B. A.
Right arrow Articles by Goodman, A. E.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., Nov 1995, 3875-3883, Vol 61, No. 11
Copyright © 1995, American Society for Microbiology

Genetic diversity and phylogeny of toxic cyanobacteria determined by DNA polymorphisms within the phycocyanin locus

BA Neilan, D Jacobs and AE Goodman
School of Microbiology and Immunology, University of New South Wales, Sydney, Australia.

Cyanobacteria are a highly diverse group in relation to form, function, and habitat. Current cyanobacterial systematics relies on the observation of minor and plastic morphological characters. Accurate and reliable delineation of toxic and bloom-forming strains of cyanobacteria has not been possible by traditional methods. We have designed general primers to the phycocyanin operon (cpc gene) and developed a PCR which allows the amplification of a region of this gene, including a variable intergenic spacer sequence. Because of the specificity of this PCR for cyanobacterial isolates, the assay is appropriate for the rapid and reliable identification of strains in freshwater samples. Successive restriction endonuclease digestion of this amplification product, with a total of nine enzymes, yielded many identifying DNA profiles specific to the various taxonomic levels of cyanobacteria. The restriction enzyme profiles for MspI, RsaI, and TaqI were conserved for strains within each of the eight genera (40 strains) studied and clearly discriminated among these genera. Intrageneric delineation of strains was revealed by the enzymes AluI, CfoI, and HaeIII for members of the genus Microcystis, while strains of genus Anabaena were differentiated by the digestion patterns provided by AluI, CfoI, and ScrFI. Phenetic and cladistic analyses of the data were used to infer the genetic relatedness and evolution of toxic and bloom- forming cyanobacteria.


This article has been cited by other articles:

  • Stuken, A., Campbell, R. J., Quesada, A., Sukenik, A., Dadheech, P. K., Wiedner, C. (2009). Genetic and morphologic characterization of four putative cylindrospermopsin producing species of the cyanobacterial genera Anabaena and Aphanizomenon. J PLANKTON RES 31: 465-480 [Abstract] [Full Text]  
  • Berrendero, E., Perona, E., Mateo, P. (2008). Genetic and morphological characterization of Rivularia and Calothrix (Nostocales, Cyanobacteria) from running water. Int. J. Syst. Evol. Microbiol. 58: 447-460 [Abstract] [Full Text]  
  • Tanabe, Y., Kasai, F., Watanabe, M. M. (2007). Multilocus sequence typing (MLST) reveals high genetic diversity and clonal population structure of the toxic cyanobacterium Microcystis aeruginosa. Microbiology 153: 3695-3703 [Abstract] [Full Text]  
  • Nakasugi, K., Alexova, R., Svenson, C. J., Neilan, B. A. (2007). Functional Analysis of PilT from the Toxic Cyanobacterium Microcystis aeruginosa PCC 7806. J. Bacteriol. 189: 1689-1697 [Abstract] [Full Text]  
  • Kim, S.-G., Rhee, S.-K., Ahn, C.-Y., Ko, S.-R., Choi, G.-G., Bae, J.-W., Park, Y.-H., Oh, H.-M. (2006). Determination of Cyanobacterial Diversity during Algal Blooms in Daechung Reservoir, Korea, on the Basis of cpcBA Intergenic Spacer Region Analysis.. Appl. Environ. Microbiol. 72: 3252-3258 [Abstract] [Full Text]  
  • Copp, J. N., Neilan, B. A. (2006). The Phosphopantetheinyl Transferase Superfamily: Phylogenetic Analysis and Functional Implications in Cyanobacteria. Appl. Environ. Microbiol. 72: 2298-2305 [Abstract] [Full Text]  
  • Ehrenreich, I. M., Waterbury, J. B., Webb, E. A. (2005). Distribution and Diversity of Natural Product Genes in Marine and Freshwater Cyanobacterial Cultures and Genomes. Appl. Environ. Microbiol. 71: 7401-7413 [Abstract] [Full Text]  
  • Wilson, A. E., Sarnelle, O., Neilan, B. A., Salmon, T. P., Gehringer, M. M., Hay, M. E. (2005). Genetic Variation of the Bloom-Forming Cyanobacterium Microcystis aeruginosa within and among Lakes: Implications for Harmful Algal Blooms. Appl. Environ. Microbiol. 71: 6126-6133 [Abstract] [Full Text]  
  • Moffitt, M. C., Neilan, B. A. (2004). Characterization of the Nodularin Synthetase Gene Cluster and Proposed Theory of the Evolution of Cyanobacterial Hepatotoxins. Appl. Environ. Microbiol. 70: 6353-6362 [Abstract] [Full Text]  
  • Thacker, R. W., Paul, V. J. (2004). Morphological, Chemical, and Genetic Diversity of Tropical Marine Cyanobacteria Lyngbya spp. and Symploca spp. (Oscillatoriales). Appl. Environ. Microbiol. 70: 3305-3312 [Abstract] [Full Text]  
  • Welker, M., Brunke, M., Preussel, K., Lippert, I., von Dohren, H. (2004). Diversity and distribution of Microcystis (Cyanobacteria) oligopeptide chemotypes from natural communities studied by single-colony mass spectrometry. Microbiology 150: 1785-1796 [Abstract] [Full Text]  
  • Kurmayer, R., Christiansen, G., Chorus, I. (2003). The Abundance of Microcystin-Producing Genotypes Correlates Positively with Colony Size in Microcystis sp. and Determines Its Microcystin Net Production in Lake Wannsee. Appl. Environ. Microbiol. 69: 787-795 [Abstract] [Full Text]  
  • Baker, J. A., Entsch, B., Neilan, B. A., McKay, D. B. (2002). Monitoring Changing Toxigenicity of a Cyanobacterial Bloom by Molecular Methods. Appl. Environ. Microbiol. 68: 6070-6076 [Abstract] [Full Text]  
  • Dyble, J., Paerl, H. W., Neilan, B. A. (2002). Genetic Characterization of Cylindrospermopsis raciborskii (Cyanobacteria) Isolates from Diverse Geographic Origins Based on nifH and cpcBA-IGS Nucleotide Sequence Analysis. Appl. Environ. Microbiol. 68: 2567-2571 [Abstract] [Full Text]  
  • Iteman, I., Rippka, R., Tandeau de Marsac, N., Herdman, M. (2002). rDNA analyses of planktonic heterocystous cyanobacteria, including members of the genera Anabaenopsis and Cyanospira. Microbiology 148: 481-496 [Abstract] [Full Text]  
  • Dittmann, E., Erhard, M., Kaebernick, M., Scheler, C., Neilan, B. A., von Dohren, H., Borner, T. (2001). Altered expression of two light-dependent genes in a microcystin-lacking mutant of Microcystis aeruginosa PCC 7806. Microbiology 147: 3113-3119 [Abstract] [Full Text]  
  • Laamanen, M. J., Gugger, M. F., Lehtimaki, J. M., Haukka, K., Sivonen, K. (2001). Diversity of Toxic and Nontoxic Nodularia Isolates (Cyanobacteria) and Filaments from the Baltic Sea. Appl. Environ. Microbiol. 67: 4638-4647 [Abstract] [Full Text]  
  • Tillett, D., Parker, D. L., Neilan, B. A. (2001). Detection of Toxigenicity by a Probe for the Microcystin Synthetase A Gene (mcyA) of the Cyanobacterial Genus Microcystis: Comparison of Toxicities with 16S rRNA and Phycocyanin Operon (Phycocyanin Intergenic Spacer) Phylogenies. Appl. Environ. Microbiol. 67: 2810-2818 [Abstract] [Full Text]  
  • Barker, G. L. A., Handley, B. A., Vacharapiyasophon, P., Stevens, J. R., Hayes, P. K. (2000). Allele-specific PCR shows that genetic exchange occurs among genetically diverse Nodularia (Cyanobacteria) filaments in the Baltic Sea. Microbiology 146: 2865-2875 [Abstract] [Full Text]  
  • Beltran, E. C., Neilan, B. A. (2000). Geographical Segregation of the Neurotoxin-Producing Cyanobacterium Anabaena circinalis. Appl. Environ. Microbiol. 66: 4468-4474 [Abstract] [Full Text]  
  • Wilson, K. M., Schembri, M. A., Baker, P. D., Saint, C. P. (2000). Molecular Characterization of the Toxic Cyanobacterium Cylindrospermopsis raciborskii and Design of a Species-Specific PCR. Appl. Environ. Microbiol. 66: 332-338 [Abstract] [Full Text]  
  • Beard, S. J., Handley, B. A., Hayes, P. K., Walsby, A. E. (1999). The diversity of gas vesicle genes in Planktothrix rubescens from Lake Zurich. Microbiology 145: 2757-2768 [Abstract] [Full Text]  
  • Neilan, B. A., Dittmann, E., Rouhiainen, L., Bass, R. A., Schaub, V., Sivonen, K., Börner, T. (1999). Nonribosomal Peptide Synthesis and Toxigenicity of Cyanobacteria. J. Bacteriol. 181: 4089-4097 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»