This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gardella, R. S.
Right arrow Articles by Del Giudice, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gardella, R. S.
Right arrow Articles by Del Giudice, R. A.
Agricola
Right arrow Articles by Gardella, R. S.
Right arrow Articles by Del Giudice, R. A.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., 05 1995, 1976-1979, Vol 61, No. 5
Copyright © 1995, American Society for Microbiology

Growth of Mycoplasma hyorhinis cultivar alpha on semisynthetic medium

RS Gardella and RA Del Giudice
Mycoplasma Laboratory, PRI/DynCorp, NCI-Frederick Cancer Research and Development Center, Maryland 21702, USA.

Serial passage of Mycoplasma hyorhinis cultivar alpha (formerly noncultivable strains) has been accomplished in modified CMRL-1066 medium with fetal bovine serum. In modified CMRL-1066 liquid medium, cultivar alpha strains grow at a similar rate and to equivalent titers when compared with BTS-7, the type strain of the species. Further experiments with BTS-7 demonstrate that the extent of growth obtained in the semidefined medium was comparable to growth in conventional mycoplasma medium. M. hyorhinis strains, including cultivar alpha strains, grow in serial passage when fetal bovine serum is replaced with bovine serum albumin, palmitic acid, and cholesterol. The results of these studies show that M. hyorhinis cultivar alpha strains are not nutritionally more fastidious than other mycoplasmas but that they are noncultivable on standard mycoplasma media because they are sensitive to high levels of inhibition activity by medium components.


This article has been cited by other articles:

  • Volokhov, D. V., Kong, H., George, J., Anderson, C., Chizhikov, V. E. (2008). Biological Enrichment of Mycoplasma Agents by Cocultivation with Permissive Cell Cultures. Appl. Environ. Microbiol. 74: 5383-5391 [Abstract] [Full Text]  
  • Razin, S., Yogev, D., Naot, Y. (1998). Molecular Biology and Pathogenicity of Mycoplasmas. Microbiol. Mol. Biol. Rev. 62: 1094-1156 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»