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Appl. Environ. Microbiol., May 1995, 2020-2022, Vol 61, No. 5
Copyright © 1995, American Society for Microbiology

Emergence of nylon oligomer degradation enzymes in Pseudomonas aeruginosa PAO through experimental evolution

ID Prijambada, S Negoro, T Yomo and I Urabe
Department of Biotechnology, Faculty of Engineering, Osaka University, Japan.

Through selective cultivation with 6-aminohexanoate linear dimer, a by- product of nylon-6 manufacture, as the sole source of carbon and nitrogen, Pseudomonas aeruginosa PAO, which initially has no enzyme activity to degrade this xenobiotic compound, was successfully expanded in its metabolic ability. Two new enzyme activities, 6-aminohexanoate cyclic dimer hydrolase and 6-aminohexanoate dimer hydrolase, were detected in the adapted strains.