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Appl. Environ. Microbiol., 05 1995, 2023-2026, Vol 61, No. 5
Copyright © 1995, American Society for Microbiology

Cloning and characterization of the abortive infection genetic determinant abiD isolated from pBF61 of Lactococcus lactis subsp. lactis KR5

LA McLandsborough, KM Kolaetis, T Requena and LL McKay
Department of Food Science and Nutrition, University of Minnesota, St. Paul 55108, USA.

A 6.3-kb fragment from pBF61 in Lactococcus lactis subsp. lactis KR5 was cloned and found to confer an abortive phage infection (Abi+) phenotype exhibiting a reduction in efficiency of plating and plaque size for small isometric- and prolate-headed bacteriophages sk1 and c2, respectively, and to produce a 10-fold decrease in c2 phage burst size. Phage adsorption was not significantly reduced. An open reading frame of 1,098 bp was sequenced and designated abiD. Tn5 mutagenesis confirmed that abiD was required for the Abi+ phenotype.

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