This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hazeleger, W. C.
Right arrow Articles by Abee, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hazeleger, W. C.
Right arrow Articles by Abee, T.
Agricola
Right arrow Articles by Hazeleger, W. C.
Right arrow Articles by Abee, T.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., 07 1995, 2713-2719, Vol 61, No. 7
Copyright © 1995, American Society for Microbiology

Temperature-dependent membrane fatty acid and cell physiology changes in coccoid forms of Campylobacter jejuni

WC Hazeleger, JD Janse, PM Koenraad, RR Beumer, FM Rombouts and T Abee
Department of Food Science, Wageningen Agricultural University, The Netherlands.

The effect of temperature and the availability of nutrients on the transition of spiral Campylobacter jejuni cells to coccoid forms was investigated. Ageing of spiral C. jejuni cells in either nutrient-poor or nutrient-rich environments resulted in the formation of nonculturable coccoid cells at 4, 12, and 25 degrees C after different periods, with the cells incubated at 4 degrees C in nutrient-deficient media remaining culturable the longest. To study the phenomenon, ATP levels, protein profiles, and fatty acid compositions were monitored under conditions where the transition from spiral to coccoid cells occurred. During storage, the levels of intracellular ATP were highest in cells incubated at low temperatures (4 and 12 degrees C) and remained constant after a small initial decrease. During the transformation from spiral to coccoid forms, no alteration in protein profiles could be detected; indeed, inhibition of protein synthesis by chloramphenicol did not influence the transition. Furthermore, DNA damage by gamma irradiation had no effect on the process. Membrane fatty acid composition of cocci formed at low temperatures was found to be almost identical to that of spiral cells, whereas that of cocci formed at 25 degrees C was clearly different. Combining these results, it is concluded that the formation of cocci is not an active process. However, distinctions between cocci formed at different temperatures were observed. Cocci formed at 4 degrees C show characteristics comparable to those of spirals, and these cocci may well play a role in the contamination cycle of C. jejuni.(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:

  • Hughes, R.-A., Hallett, K., Cogan, T., Enser, M., Humphrey, T. (2009). The Response of Campylobacter jejuni to Low Temperature Differs from That of Escherichia coli. Appl. Environ. Microbiol. 75: 6292-6298 [Abstract] [Full Text]  
  • Budding, A. E., Ingham, C. J., Bitter, W., Vandenbroucke-Grauls, C. M., Schneeberger, P. M. (2009). The Dienes Phenomenon: Competition and Territoriality in Swarming Proteus mirabilis. J. Bacteriol. 191: 3892-3900 [Abstract] [Full Text]  
  • Wright, J. A., Grant, A. J., Hurd, D., Harrison, M., Guccione, E. J., Kelly, D. J., Maskell, D. J. (2009). Metabolite and transcriptome analysis of Campylobacter jejuni in vitro growth reveals a stationary-phase physiological switch. Microbiology 155: 80-94 [Abstract] [Full Text]  
  • Mouwen, D. J. M., Weijtens, M. J. B. M., Capita, R., Alonso-Calleja, C., Prieto, M. (2005). Discrimination of Enterobacterial Repetitive Intergenic Consensus PCR Types of Campylobacter coli and Campylobacter jejuni by Fourier Transform Infrared Spectroscopy. Appl. Environ. Microbiol. 71: 4318-4324 [Abstract] [Full Text]  
  • Axelsson-Olsson, D., Waldenstrom, J., Broman, T., Olsen, B., Holmberg, M. (2005). Protozoan Acanthamoeba polyphaga as a Potential Reservoir for Campylobacter jejuni. Appl. Environ. Microbiol. 71: 987-992 [Abstract] [Full Text]  
  • Kelly, A. F., Martinez-Rodriguez, A., Bovill, R. A., Mackey, B. M. (2003). Description of a "Phoenix" Phenomenon in the Growth of Campylobacter jejuni at Temperatures Close to the Minimum for Growth. Appl. Environ. Microbiol. 69: 4975-4978 [Abstract] [Full Text]  
  • Chaveerach, P., ter Huurne, A. A. H. M., Lipman, L. J. A., van Knapen, F. (2003). Survival and Resuscitation of Ten Strains of Campylobacter jejuni and Campylobacter coli under Acid Conditions. Appl. Environ. Microbiol. 69: 711-714 [Abstract] [Full Text]  
  • Nilsson, H.-O., Blom, J., Al-Soud, W. A., Ljungh, A., Andersen, L. P., Wadstrom, T. (2002). Effect of Cold Starvation, Acid Stress, and Nutrients on Metabolic Activity of Helicobacter pylori. Appl. Environ. Microbiol. 68: 11-19 [Abstract] [Full Text]  
  • Chan, K. F., Le Tran, H., Kanenaka, R. Y., Kathariou, S. (2001). Survival of Clinical and Poultry-Derived Isolates of Campylobacter jejuni at a Low Temperature (4{degrees}C). Appl. Environ. Microbiol. 67: 4186-4191 [Abstract] [Full Text]  
  • Kelly, A. F., Park, S. F., Bovill, R., Mackey, B. M. (2001). Survival of Campylobacter jejuni during Stationary Phase: Evidence for the Absence of a Phenotypic Stationary-Phase Response. Appl. Environ. Microbiol. 67: 2248-2254 [Abstract] [Full Text]  
  • Reezal, A., McNeil, B., Anderson, J. G. (1998). Effect of Low-Osmolality Nutrient Media on Growth and Culturability of Campylobacter Species. Appl. Environ. Microbiol. 64: 4643-4649 [Abstract] [Full Text]  
  • Hazeleger, W. C., Wouters, J. A., Rombouts, F. M., Abee, T. (1998). Physiological Activity of Campylobacter jejuni Far below the Minimal Growth Temperature. Appl. Environ. Microbiol. 64: 3917-3922 [Abstract] [Full Text]  
  • Höller, C., Witthuhn, D., Janzen-Blunck, B. (1998). Effect of Low Temperatures on Growth, Structure, and Metabolism of Campylobacter coli SP10. Appl. Environ. Microbiol. 64: 581-587 [Abstract] [Full Text]  
  • Buswell, C. M., Herlihy, Y. M., Lawrence, L. M., McGuiggan, J. T. M., Marsh, P. D., Keevil, C. W., Leach, S. A. (1998). Extended Survival and Persistence of Campylobacter spp. in Water and Aquatic Biofilms and Their Detection by Immunofluorescent-Antibody and -rRNA Staining. Appl. Environ. Microbiol. 64: 733-741 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»