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Appl. Environ. Microbiol., Jul 1995, 2759-2761, Vol 61, No. 7
Copyright © 1995, American Society for Microbiology

DNA fingerprinting by pulsed-field gel electrophoresis to investigate a nosocomial pneumonia caused by Legionella bozemanii serogroup 1

PC Luck, JH Helbig, HJ Hagedorn and W Ehret
Institut fur Medizinische Mikrobiologie und Hygiene, Technische Universitat Dresden, Germany.

We typed 18 isolates of Legionella bozemanii obtained from clinical and environmental sources by pulsed-field gel electrophoresis. Each of the unrelated strains showed individual restriction patterns of the genomic DNA when either the SfiI or NotI restriction enzyme was used. One strain isolated from a patient with nosocomial legionellosis and two strains from the corresponding hospital water supply were indistinguishable, arguing for a transmission of L. bozemanii from the water supply to the patient. In conclusion, macrorestriction analysis is a valuable tool for studies of the molecular epidemiology of L. bozemanii.


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