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Appl. Environ. Microbiol., 08 1995, 3169-3171, Vol 61, No. 8
Copyright © 1995, American Society for Microbiology

Cloning, nucleotide sequencing, and expression of an opine dehydrogenase gene from Arthrobacter sp. strain 1C

T Dairi and Y Asano
Biotechnology Research Center, Toyama Prefectural University, Japan.

The gene coding for opine dehydrogenase from Arthrobacter sp. strain 1C was cloned onto plasmid pBluescript KS(-), and the nucleotide sequence of the 1,077-bp open reading frame consisting of 359 codons was identified as the odh gene. Transformed Escherichia coli cells overproduced NAD+-dependent opine dehydrogenase under control of the promoter of the lac gene on pBluescript KS(-).