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Appl. Environ. Microbiol., 08 1995, 3169-3171, Vol 61, No. 8
T Dairi and Y Asano
The gene coding for opine dehydrogenase from Arthrobacter sp. strain 1C was
cloned onto plasmid pBluescript KS(-), and the nucleotide sequence of the
1,077-bp open reading frame consisting of 359 codons was identified as the
odh gene. Transformed Escherichia coli cells overproduced NAD+-dependent
opine dehydrogenase under control of the promoter of the lac gene on
pBluescript KS(-).
Copyright © 1995, American Society for Microbiology
Cloning, nucleotide sequencing, and expression of an opine dehydrogenase gene from Arthrobacter sp. strain 1C
Biotechnology Research Center, Toyama Prefectural University, Japan.
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