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Appl. Environ. Microbiol., Aug 1995, 3172-3176, Vol 61, No. 8
Copyright © 1995, American Society for Microbiology

Characterization of plasmid-encoded citrate permease (citP) genes from Leuconostoc species reveals high sequence conservation with the Lactococcus lactis citP gene

EE Vaughan, S David, A Harrington, C Daly, GF Fitzgerald and WM De Vos
Department of Biophysical Chemistry, Netherlands Institute for Dairy Research (NIZO), Ede.

The citrate permease determinant (citP) in several Leuconostoc strains was demonstrated to be plasmid encoded by curing experiments and hybridization studies with a DNA fragment containing the citP gene from Lactococcus lactis subsp. lactis biovar diacetylactis NCDO176. Cloning and nucleotide sequence analysis of Leuconostoc lactis NZ6070 citP revealed almost complete identity to lactococcal citP.


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