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Appl. Environ. Microbiol., Aug 1995, 3189-3191, Vol 61, No. 8
Copyright © 1995, American Society for Microbiology

Selection and Characterization of Aspartokinase Feedback-Insensitive Mutants of Azotobacter vinelandii

CR Ekechukwu, TA Burns and T Melton
Department of Microbiology, North Carolina State University, Raleigh, North Carolina 27695

Aspartokinase feedback-insensitive mutants of Azotobacter vinelandii were selected as resistant to l-threonine, (beta)-hydroxynorvaline, or S-(2-aminoethyl)-l-cysteine. l-Threonine-resistant strains were classified into three groups based on their ability to transport l-threonine and their growth response to O-methylthreonine and (beta)-hydroxynorvaline. Most of the mutants were transport defective; however, some were desensitized to feedback regulation.

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