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Appl. Environ. Microbiol., 09 1995, 3476-3478, Vol 61, No. 9
Copyright © 1995, American Society for Microbiology

Nested PCR method for rapid and sensitive detection of Vibrio vulnificus in fish, sediments, and water

CR Arias, E Garay and R Aznar
Departamento de Microbiologia, Universitat de Valencia, Burjassot, Spain.

A nested PCR for the detection of Vibrio vulnificus in fish farms was developed as an alternative to cultural methods by using universal primers flanking the V. vulnificus-specific sequences directed against 23S rRNA genes. This specific assay detected 10 fg of DNA or 12 to 120 cells in artificially inoculated samples without enrichment and within 24 h.


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