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Appl. Environ. Microbiol., Jan 1996, 266-268, Vol 62, No. 1
Copyright © 1996, American Society for Microbiology

Optimal Renewal Rate and Nutrient Concentration for the Production of the Marine Microalga Phaeodactylum tricornutum in Semicontinuous Cultures

J Fabregas, M Patino, ED Morales, B Cordero and A Otero
Departamento de Microbiologia, Facultad de Farmacia, Universidad de Santiago, Santiago, Spain

A factorial experiment comprising six nutrient concentrations and six renewal rates was set to optimize semicontinuous cultures of Phaeodactylum tricornutum. Maximal cell density, 85 x 10(sup6) cells ml(sup-1), was obtained with 8 and 16 mmol of N per liter and a renewal rate of 10%. Protein stabilized at about 60% of the organic fraction for nitrogen-sufficient cultures. Both lipids and carbohydrates were stored as energetic reserves.




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