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Appl. Environ. Microbiol., Jan 1996, 275-278, Vol 62, No. 1
Copyright © 1996, American Society for Microbiology

Detection of specific bacterial cells with 2-hydroxy-3-naphthoic acid- 2'-phenylanilide phosphate and fast red TR in situ hybridization

N Yamaguchi, S Inaoka, K Tani, T Kenzaka and M Nasu
Faculty of Pharmaceutical Sciences, Osaka University, Japan.

An in situ hybridization technique with HNPP (2-hydroxy-3-naphthoic acid-2'-phenylanilide phosphate) and Fast Red TR was used to detect specific bacterial cells at the single-cell level. By this technique, the fluorescent signals of target bacterial cells were up to eight times more intense than those of standard fluorescence in situ hybridization with mono-fluorescein isothiocyanate-labeled oligonucleotide probes. This novel HNPP-Fast Red TR whole-cell hybridization technique is available for the identification of small or low-rRNA-content bacterial cells in the natural environment.


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