This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wolfersberger, M. G.
Right arrow Articles by Dean, D. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wolfersberger, M. G.
Right arrow Articles by Dean, D. H.
Agricola
Right arrow Articles by Wolfersberger, M. G.
Right arrow Articles by Dean, D. H.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., 01 1996, 279-282, Vol 62, No. 1
Copyright © 1996, American Society for Microbiology

Site-directed mutations in the third domain of Bacillus thuringiensis delta-endotoxin CryIAa affect its ability to increase the permeability of Bombyx mori midgut brush border membrane vesicles

MG Wolfersberger, XJ Chen and DH Dean
Department of Biology, Temple University, Philadelphia, Pennsylvania 19122, USA.

A series of mutant Bacillus thuringiensis CryIAa delta-endotoxin proteins was prepared by replacing the first, second, and last arginine residues of the conserved third-domain sequence, R-521 YRVRIR-527, with other amino acids. The stable mutant proteins were bioassayed against Bombyx mori larvae and found to all be approximately half as active as wild-type CryIAa. The toxins were also tested by means of a light- scattering assay for their ability to increase permeability of larval B. mori midgut brush border membrane vesicles. Three of the mutant toxins were as active as the wild-type toxin in the vesicle permeability assay.


This article has been cited by other articles:

  • Boonserm, P., Mo, M., Angsuthanasombat, C., Lescar, J. (2006). Structure of the Functional Form of the Mosquito Larvicidal Cry4Aa Toxin from Bacillus thuringiensis at a 2.8-Angstrom Resolution. J. Bacteriol. 188: 3391-3401 [Abstract] [Full Text]  
  • Liu, X. S., Dean, D. H. (2006). Redesigning Bacillus thuringiensis Cry1Aa toxin into a mosquito toxin. Protein Eng Des Sel 19: 107-111 [Abstract] [Full Text]  
  • Atsumi, S., Mizuno, E., Hara, H., Nakanishi, K., Kitami, M., Miura, N., Tabunoki, H., Watanabe, A., Sato, R. (2005). Location of the Bombyx mori Aminopeptidase N Type 1 Binding Site on Bacillus thuringiensis Cry1Aa Toxin. Appl. Environ. Microbiol. 71: 3966-3977 [Abstract] [Full Text]  
  • Masson, L., Tabashnik, B. E., Mazza, A., Prefontaine, G., Potvin, L., Brousseau, R., Schwartz, J.-L. (2002). Mutagenic Analysis of a Conserved Region of Domain III in the Cry1Ac Toxin of Bacillus thuringiensis. Appl. Environ. Microbiol. 68: 194-200 [Abstract] [Full Text]  
  • Lee, M. K., You, T. H., Gould, F. L., Dean, D. H. (1999). Identification of Residues in Domain III of Bacillus thuringiensis Cry1Ac Toxin That Affect Binding and Toxicity. Appl. Environ. Microbiol. 65: 4513-4520 [Abstract] [Full Text]  
  • Rang, C., Vachon, V., de Maagd, R. A., Villalon, M., Schwartz, J.-L., Bosch, D., Frutos, R., Laprade, R. (1999). Interaction between Functional Domains of Bacillus thuringiensis Insecticidal Crystal Proteins. Appl. Environ. Microbiol. 65: 2918-2925 [Abstract] [Full Text]  
  • Ballester, V., Granero, F., de Maagd, R. A., Bosch, D., Ménsua, J. L., Ferré, J. (1999). Role of Bacillus thuringiensis Toxin Domains in Toxicity and Receptor Binding in the Diamondback Moth. Appl. Environ. Microbiol. 65: 1900-1903 [Abstract] [Full Text]  
  • Luo, K., Banks, D., Adang, M. J. (1999). Toxicity, Binding, and Permeability Analyses of Four Bacillus thuringiensis Cry1 delta -Endotoxins Using Brush Border Membrane Vesicles of Spodoptera exigua and Spodoptera frugiperda. Appl. Environ. Microbiol. 65: 457-464 [Abstract] [Full Text]  
  • Schnepf, E., Crickmore, N., Van Rie, J., Lereclus, D., Baum, J., Feitelson, J., Zeigler, D. R., Dean, D. H. (1998). Bacillus thuringiensis and Its Pesticidal Crystal Proteins. Microbiol. Mol. Biol. Rev. 62: 775-806 [Abstract] [Full Text]  
  • Kasman, L. M., Lukowiak, A. A., Garczynski, S. F., McNall, R. J., Youngman, P., Adang, M. J. (1998). Phage Display of a Biologically Active Bacillus thuringiensis Toxin. Appl. Environ. Microbiol. 64: 2995-3003 [Abstract] [Full Text]  
  • Escriche, B., De Decker, N., Van Rie, J., Jansens, S., Van Kerkhove, E. (1998). Changes in Permeability of Brush Border Membrane Vesicles from Spodoptera littoralis Midgut Induced by Insecticidal Crystal Proteins from Bacillus thuringiensis. Appl. Environ. Microbiol. 64: 1563-1565 [Abstract] [Full Text]  
  • Rajamohan, F., Alzate, O., Cotrill, J. A., Curtiss, A., Dean, D. H. (1996). Protein engineering of Bacillus thuringiensis delta -endotoxin: Mutations at domain II of CryIAb enhance receptor affinity and toxicity toward gypsy moth larvae. Proc. Natl. Acad. Sci. USA 93: 14338-14343 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»