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Appl. Environ. Microbiol., Jan 1996, 300-303, Vol 62, No. 1
Copyright © 1996, American Society for Microbiology

Critical Evaluation of Membrane Supports for Use in Quantitative Hybridizations

L Raskin, WC Capman, MD Kane, BE Rittmann and DA Stahl
Environmental Engineering and Science, Department of Civil Engineering, Newmark Civil Engineering Laboratory, Department of Veterinary Pathobiology, and Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, and Department of Civil Engineering, Technological Institute, Northwestern University, Evanston, Illinois 60208

The quantification of 16S rRNA by oligonucleotide probe hybridization was investigated with MagnaGraph (Micron Separation, Inc. [MSI]), Magna Charge (MSI), Magna (MSI), Immobilon-N (Millipore Corporation), and Nytran (Schleicher & Schuell, Inc.) membranes as supports for nucleic acid immobilization. The levels of detectability provided by the Magna Charge and Immobilon-N membranes were 20 to 50 times better than those obtained with the MagnaGraph, Magna, and Nytran membranes. The variability of the signal response for individual membranes ranged from 10 to 50%, with the Magna Charge and Immobilon-N membranes demonstrating the lowest variability.

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