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Appl. Environ. Microbiol., Oct 1996, 3901-3904, Vol 62, No. 10
Copyright © 1996, American Society for Microbiology

Identification of a functionally important population in phenol- digesting activated sludge with antisera raised against isolated bacterial strains

K Watanabe and S Hino
Fundamental Research Laboratories, Tonen Corporation, Saitama, Japan. LDJ03665@niftyserve.or.jp

Antisera were raised against nine strains which had been isolated from phenol-acclimated oil refinery activated sludge. Although several antisera reacted significantly with the activated sludge during a period of adaptation to phenol, only an antiserum against one of the isolates, Alcaligenes sp. E2, reacted with the activated sludge after the adaptation period. A kinetic pattern of phenol-oxygenating activity of the activated sludge after the adaptation period was similar to that of strain E2. These results suggest that a functionally important population in the phenol-digesting activated sludge was serologically identified.


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