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Appl. Environ. Microbiol., Nov 1996, 4220-4223, Vol 62, No. 11
Copyright © 1996, American Society for Microbiology

Purification and characterization of enterocin 4, a bacteriocin produced by Enterococcus faecalis INIA 4

HM Joosten, M Nunez, B Devreese, J Van Beeumen and JD Marugg
Departamento de Tecnologia de Alimentos, CIT-INIA, Madrid, Spain. han.joosten@chlsnr.nestrd.ch

A simple two-step procedure was developed to obtain pure enterocin 4, a bacteriocin produced by Enterococcus faecalis INIA 4. Chemical and genetic characterization revealed that the primary structure of enterocin 4 is identical to that of peptide antibiotic AS-48 from Enterococcus faecalis S-48. In contrast to the reported inhibitory spectrum of AS-48, enterocin 4 displayed no activity against gram- negative bacteria.


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