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Appl. Environ. Microbiol., Feb 1996, 380-384, Vol 62, No. 2
Copyright © 1996, American Society for Microbiology

Growth-Associated Production of Poly(3-Hydroxyvalerate) from n-Pentanol by a Methylotrophic Bacterium, Paracoccus denitrificans

T Yamane, X Chen and S Ueda
Laboratory of Molecular Biotechnology, Department of Applied Biological Sciences, Faculty of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464-01, and Laboratory of Applied Microbiology, Department of Bioproductive Sciences, Faculty of Agriculture, Utsunomiya University, 350 Mine-machi, Utsunomiya 321, Japan

Paracoccus denitrificans accumulated a polyester in its cells during growth on n-pentanol. The composition of the polyester varied during the cultivation: the level of the 3-hydroxyvalerate unit in the polyester increased, and eventually a homopolymeric poly(3-hydroxyvalerate) [P(3HV)] accumulated to an amount 22 to 24% of the cell dry weight. Growth-associated polyester synthesis was considerably affected by n-pentanol when its concentration was controlled at several levels. Maximum accumulation of the polyester was obtained at 0.02% (vol/vol). Physical and mechanical characteristics of the P(3HV) were determined and compared with those of other homo- and copolyesters. The P(3HV) was dextrorotatory and had number-averaged and weight-averaged molecular masses of 128,000 and 888,000 Da, respectively, with a rate of polydispersity of 6.93. The level of tensile strength of the P(3HV) was lower, and its extension to break was higher than that of the poly(3-hydroxybutyrate) homopolyester.


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