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Appl. Environ. Microbiol., Feb 1996, 429-436, Vol 62, No. 2
Copyright © 1996, American Society for Microbiology

Growth Rate-Dependent Modulation of Carbon Flux through Central Metabolism and the Kinetic Consequences for Glucose-Limited Chemostat Cultures of Corynebacterium glutamicum

M Cocaign-Bousquet, A Guyonvarch and ND Lindley
Centre de Bioingenierie Gilbert Durand (URA CNRS & Lab. Ass. INRA), INSA, Complexe Scientifique de Rangueil, 31077 Toulouse cedex, and Institut de Genetique et Microbiologie, Universite Paris Sud, 91405 Orsay cedex, France

The physiological behavior of Corynebacterium glutamicum in glucose-limited chemostat cultures was examined from both growth kinetics and enzymatic viewpoints. Metabolic fluxes within the central metabolism were calculated from growth kinetics and analyzed in relation to specific enzyme activities. At high growth rates, incomplete glucose removal was observed, and this was attributed to rate-limiting capacity of the phosphotransferase system transporter and the probable contribution of a low-affinity permease uptake mechanism. The improved biomass yield observed at high growth rates was related to a shift in the profile of anaplerotic carboxylation reactions, with pyruvate carboxylase replacing malic enzyme. Phosphoenolpyruvate carboxylase, an activity often assumed to be the major anaplerotic reaction during growth of C. glutamicum on glucose, was present at only low levels and is unlikely to contribute significantly to tricarboxylic acid cycle fuelling other than at low growth rates.


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