This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lee, M. K.
Right arrow Articles by Dean, D. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, M. K.
Right arrow Articles by Dean, D. H.
Agricola
Right arrow Articles by Lee, M. K.
Right arrow Articles by Dean, D. H.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., Feb 1996, 583-586, Vol 62, No. 2
Copyright © 1996, American Society for Microbiology

Synergistic effect of the Bacillus thuringiensis toxins CryIAa and CryIAc on the gypsy moth, Lymantria dispar

MK Lee, A Curtiss, E Alcantara and DH Dean
Department of Biochemistry, Ohio State University, Columbus 43210, USA.

The insecticidal activity of toxins CryIAa, CryIAb, and CryIAc against Lymantria dispar (gypsy moth) and Bombyx mori (silkworm) was examined by force-feeding bioassays. Toxin CryIAa exhibited higher toxicity than toxins CryIAb and CryIAc for L. dispar and B. mori. To evaluate possible synergism among these toxins, bioassays were performed with mixtures of CryIAa and CryIAb, CryIAb and CryIAc, and CryIAa and CryIAc. Expected toxicity was calculated from the activity of each individual toxin and its proportion in the mixture by using the equation described by Tabashnik (B. E. Tabashnik, Appl. Environ. Microbiol. 58:3343-3346, 1992). Observed 50% growth-inhibitory doses were calculated from mixing experiments by probit analysis. In L. dispar bioassays, synergism was observed with a mixture of CryIAa and CryIAc while a mixture of CryIAa and CryIAb exhibited an antagonistic effect. No synergistic effect on B. mori was observed with any toxin combination. Voltage clamping assays of isolated L. dispar midguts also demonstrated that the mixture of CryIAa and CryIAc induced a greater slope of inhibition of short circuit current than did other toxin combinations.


This article has been cited by other articles:

  • Chen, J., Hua, G., Jurat-Fuentes, J. L., Abdullah, M. A., Adang, M. J. (2007). Synergism of Bacillus thuringiensis toxins by a fragment of a toxin-binding cadherin. Proc. Natl. Acad. Sci. USA 104: 13901-13906 [Abstract] [Full Text]  
  • Abdullah, M. A. F., Alzate, O., Mohammad, M., McNall, R. J., Adang, M. J., Dean, D. H. (2003). Introduction of Culex Toxicity into Bacillus thuringiensis Cry4Ba by Protein Engineering. Appl. Environ. Microbiol. 69: 5343-5353 [Abstract] [Full Text]  
  • Sedlak, M., Walter, T., Aronson, A. (2000). Regulation by Overlapping Promoters of the Rate of Synthesis and Deposition into Crystalline Inclusions of Bacillus thuringiensis delta -Endotoxins. J. Bacteriol. 182: 734-741 [Abstract] [Full Text]  
  • Cheng, P., Wu, L., Ziniu, Y., Aronson, A. (1999). Subspecies-Dependent Regulation of Bacillus thuringiensis Protoxin Genes. Appl. Environ. Microbiol. 65: 1849-1853 [Abstract] [Full Text]  
  • del Rincón-Castro, M. C., Barajas-Huerta, J., Ibarra, J. E. (1999). Antagonism between Cry1Ac1 and Cyt1A1 Toxins of Bacillus thuringiensis. Appl. Environ. Microbiol. 65: 2049-2053 [Abstract] [Full Text]  
  • Walter, T., Aronson, A. (1999). Specific Binding of the E2 Subunit of Pyruvate Dehydrogenase to the Upstream Region of Bacillus thuringiensis Protoxin Genes. J. Biol. Chem. 274: 7901-7906 [Abstract] [Full Text]  
  • Masson, L., Erlandson, M., Puzstai-Carey, M., Brousseau, R., Juárez-Pérez, V., Frutos, R. (1998). A Holistic Approach for Determining the Entomopathogenic Potential of Bacillus thuringiensis Strains. Appl. Environ. Microbiol. 64: 4782-4788 [Abstract] [Full Text]  
  • Schnepf, E., Crickmore, N., Van Rie, J., Lereclus, D., Baum, J., Feitelson, J., Zeigler, D. R., Dean, D. H. (1998). Bacillus thuringiensis and Its Pesticidal Crystal Proteins. Microbiol. Mol. Biol. Rev. 62: 775-806 [Abstract] [Full Text]  
  • Liu, Y.-B., Tabashnik, B. E., Moar, W. J., Smith, R. A. (1998). Synergism between Bacillus thuringiensis Spores and Toxins against Resistant and Susceptible Diamondback Moths (Plutella xylostella). Appl. Environ. Microbiol. 64: 1385-1389 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»