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Appl. Environ. Microbiol., Feb 1996, 728-731, Vol 62, No. 2
Copyright © 1996, American Society for Microbiology

Methanococcus thermolithotrophicus Isolated from North Sea Oil Field Reservoir Water

RK Nilsen and T Torsvik
Department of Microbiology, University of Bergen, Bergen, Norway

Methanococcus thermolithotrophicus ST22 was isolated from produced water of a North Sea oil field, on mineral medium with H(inf2)-CO(inf2) as the sole source of carbon and energy. The isolate grew at 17 to 62(deg)C, with an optimum at 60(deg)C. The pH range was 4.9 to 9.8, with optimal growth at pH 5.1 to 5.9; these characteristics reflected its habitat. Strain ST22 was quickly identified and distinguished from the type strain by immunoblotting.


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