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Appl. Environ. Microbiol., Apr 1996, 1444-1447, Vol 62, No. 4
Copyright © 1996, American Society for Microbiology

Recombinant protein expression at low temperatures under the transcriptional control of the major Escherichia coli cold shock promoter cspA

JA Vasina and F Baneyx
Department of Chemical Engineering, University of Washington, Seattle 98195, USA.

A transcriptional gene fusion between the cspA promoter and the lacZ gene was constructed to assess the usefulness of cold shock promoters for low-temperature protein expression. Synthesis of beta-galactosidase was efficiently repressed at 37 degrees C but rapidly induced upon transfer to the 15-to-30 degrees C range, leading to a three- to fivefold increase in specific activity relative to control cultures. Although the initial rates of beta-galactosidase accumulation at 20 degrees C were twice those measured at 15 degrees C, prolonged incubation at 20 degrees C, but not 15 degrees C, led to a dilution of activity due to repression of the promoter and cell division.

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