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Appl. Environ. Microbiol., May 1996, 1805-1807, Vol 62, No. 5
Copyright © 1996, American Society for Microbiology

Use of Colicin E3 for Biological Containment of Microorganisms

MT Munthali, KN Timmis and E Diaz
Department of Microbiology, GBF-National Research Center for Biotechnology, D-38124 Braunschweig, Germany

The genetic determinant of the lethal antibiotic colicin E3 was cloned under the control of a tightly regulated promoter in the absence of the gene for its cognate inhibitor. Combination of this killing cassette with a stringent regulatory element provided a substrate-dependent conditional suicide system that was exploited for the biological containment of a Pseudomonas putida strain. The lethality of a single gene copy and the distinct and universal cellular target of the antibiotic suggest colicin E3 as an ideal candidate for combination with other lethal functions to design highly efficient containment systems for microorganisms.


This article has been cited by other articles:

  • Cascales, E., Buchanan, S. K., Duche, D., Kleanthous, C., Lloubes, R., Postle, K., Riley, M., Slatin, S., Cavard, D. (2007). Colicin Biology. Microbiol. Mol. Biol. Rev. 71: 158-229 [Abstract] [Full Text]  
  • Torres, B., Jaenecke, S., Timmis, K. N., Garcia, J. L., Diaz, E. (2003). A dual lethal system to enhance containment of recombinant micro-organisms. Microbiology 149: 3595-3601 [Abstract] [Full Text]  


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