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Appl. Environ. Microbiol., 07 1996, 2641-2643, Vol 62, No. 7
Copyright © 1996, American Society for Microbiology

Genetic manipulation of the pathway for diacetyl metabolism in Lactococcus lactis

SR Swindell, KH Benson, HG Griffin, P Renault, SD Ehrlich and MJ Gasson
Institute of Food Research, Colney, United Kingdom.

Diacetyl is an important food flavor compound produced by certain strains of citrate-metabolizing lactic acid bacteria. Citrate is converted to pyruvate, from which diacetyl is produced via intermediate alpha-acetolactate. This paper reports the cloning and analysis of the gene (aldB) encoding alpha-acetolactate decarboxylase from Lactococcus lactis MG1363. Deletion of the MG1363 chromosomal aldB gene was achieved by double crossover homologous recombination. The mutant strain was found to produce diacetyl; alpha-acetolactate decarboxylase activity was eliminated. Overexpression of the cloned ilvBN genes (encoding an alpha-acetolactate synthase) in the aldB deletion strain produced even higher levels of alpha-acetolactate, acetoin, and diacetyl.


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