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Appl. Environ. Microbiol., 07 1996, 2647-2650, Vol 62, No. 7
Copyright © 1996, American Society for Microbiology

Recruitment and expression of toluene/trichloroethylene biodegradation genes in bacteria native to deep-subsurface sediments

MF Romine and FJ Brockman
Pacific Northwest National Laboratory, Richland, Washington 99352, USA. mf_romine@pnl.gov

Four plasmids, each encoding a combination of either an Escherichia coli or Pseudomonas putida promoter and either toluene dioxygenase or toluene monooxygenase, were electroporated into five bacterial strains isolated from sediments found at depths of 91 to 295 m. Four of these engineered bacterial strains demonstrated both toluene and trichloroethylene degradation activities.




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