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Appl. Environ. Microbiol., Aug 1996, 3050-3052, Vol 62, No. 8
Copyright © 1996, American Society for Microbiology

Quantification of Dehalospirillum multivorans in Mixed-Culture Biofilms with an Enzyme-Linked Immunosorbent Assay

P Bauer-Kreisel, M Eisenbeis and H Scholz-Muramatsu
Department of Biology, Institute for Sanitary Engineering, University of Stuttgart, D-70569 Stuttgart, Germany

A fast, highly selective and sensitive method to quantify specific biomasses in mixed-culture biofilms is described. It consists of detachment of a biofilm from its support material, resolution of the detached biofilm flocs in order to separate the enclosed cells and antigens, and quantification of specific biomass by an enzyme-linked immunosorbent assay.


This article has been cited by other articles:

  • Morris, C. E., Monier, J.-M., Jacques, M.-A. (1998). A Technique To Quantify the Population Size and Composition of the Biofilm Component in Communities of Bacteria in the Phyllosphere. Appl. Environ. Microbiol. 64: 4789-4795 [Abstract] [Full Text]  


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