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Appl. Environ. Microbiol., 08 1996, 3053-3056, Vol 62, No. 8
Copyright © 1996, American Society for Microbiology

Isolation of a beta-tubulin gene from Fusarium moniliforme that confers cold-sensitive benomyl resistance

K Yan and MB Dickman
Department of Plant Pathology, University of Nebraska, Lincoln 68583- 0722, USA.

A beta-tubulin gene from a UV-irradiated benomyl-resistant mutant of Fusarium moniliforme was isolated, cloned, and sequenced. The gene encodes a 446-amino-acid polypeptide with homology to other fungal beta- tubulins. RNA blot analysis showed expression of the gene during vegetative growth and conidial germination but no expression during conidiation. A point mutation, which likely confers benomyl resistance, has been identified in the cloned gene; this mutation results in a single amino acid substitution of asparagine for tyrosine at position 50. Expression of benomyl resistance in the mutant was also cold sensitive. Sexual crosses betweeen the mutant and a wild-type strain indicated cosegregation of benomyl resistance and cold sensitivity.

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