This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by van Hylckama Vlieg, J.
Right arrow Articles by Janssen, D. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by van Hylckama Vlieg, J.
Right arrow Articles by Janssen, D. B.
Agricola
Right arrow Articles by van Hylckama Vlieg, J.
Right arrow Articles by Janssen, D. B.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., Sep 1996, 3304-3312, Vol 62, No. 9
Copyright © 1996, American Society for Microbiology

Transformation Kinetics of Chlorinated Ethenes by Methylosinus trichosporium OB3b and Detection of Unstable Epoxides by On-Line Gas Chromatography

JET van Hylckama Vlieg, W de Koning and DB Janssen
Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, 9747 AG Groningen, The Netherlands

A rapid and accurate method for the determination of transformation kinetics of volatile organic substrates was developed. Concentrations were monitored by on-line gas chromatographic analysis of the headspace of well-mixed incubation mixtures. With this method, the kinetics of transformation of a number of C(inf1) and C(inf2) halogenated alkanes and alkenes by Methylosinus trichosporium OB3b expressing particulate methane monooxygenase or soluble methane monooxygenase (sMMO) were studied. Apparent specific first-order rate constants for cells expressing sMMO decreased in the order of dichloromethane, vinyl chloride, cis-1,2-dichloroethene, trans-1,2-dichloroethene, 1,1-dichloroethene, trichloroethene, chloroform, and 1,2-dichloroethane. During the degradation of trichloroethene, cis-1,2-dichloroethene, trans-1,2-dichloroethene, and vinyl chloride, the formation of the corresponding epoxides was observed. The epoxide of vinyl chloride and the epoxide of trichloroethene, which temporarily accumulated in the medium, were chemically degraded according to first-order kinetics, with half-lives of 78 and 21 s, respectively. Cells expressing sMMO actively degraded the epoxide of cis-1,2-dichloroethene but not the epoxide of trans-1,2-dichloroethene. Methane and acetylene inhibited degradation of the epoxide of cis-1,2-dichloroethene, indicating that sMMO was involved.


This article has been cited by other articles:

  • Halsey, K. H., Doughty, D. M., Sayavedra-Soto, L. A., Bottomley, P. J., Arp, D. J. (2007). Evidence for Modified Mechanisms of Chloroethene Oxidation in Pseudomonas butanovora Mutants Containing Single Amino Acid Substitutions in the Hydroxylase {alpha}-Subunit of Butane Monooxygenase. J. Bacteriol. 189: 5068-5074 [Abstract] [Full Text]  
  • Doughty, D. M., Sayavedra-Soto, L. A., Arp, D. J., Bottomley, P. J. (2005). Effects of Dichloroethene Isomers on the Induction and Activity of Butane Monooxygenase in the Alkane-Oxidizing Bacterium "Pseudomonas butanovora". Appl. Environ. Microbiol. 71: 6054-6059 [Abstract] [Full Text]  
  • Rui, L., Cao, L., Chen, W., Reardon, K. F., Wood, T. K. (2004). Active Site Engineering of the Epoxide Hydrolase from Agrobacterium radiobacter AD1 to Enhance Aerobic Mineralization of cis-1,2-Dichloroethylene in Cells Expressing an Evolved Toluene ortho-Monooxygenase. J. Biol. Chem. 279: 46810-46817 [Abstract] [Full Text]  
  • Scheutz, C., Mosbaek, H., Kjeldsen, P. (2004). Attenuation of Methane and Volatile Organic Compounds in Landfill Soil Covers. J. Environ. Qual. 33: 61-71 [Abstract] [Full Text]  
  • Coleman, N. V., Mattes, T. E., Gossett, J. M., Spain, J. C. (2002). Biodegradation of cis-Dichloroethene as the Sole Carbon Source by a {beta}-Proteobacterium. Appl. Environ. Microbiol. 68: 2726-2730 [Abstract] [Full Text]  
  • Canada, K. A., Iwashita, S., Shim, H., Wood, T. K. (2002). Directed Evolution of Toluene ortho-Monooxygenase for Enhanced 1-Naphthol Synthesis and Chlorinated Ethene Degradation. J. Bacteriol. 184: 344-349 [Abstract] [Full Text]  
  • Dinkla, I. J. T., Gabor, E. M., Janssen, D. B. (2001). Effects of Iron Limitation on the Degradation of Toluene by Pseudomonas Strains Carrying the TOL (pWWO) Plasmid. Appl. Environ. Microbiol. 67: 3406-3412 [Abstract] [Full Text]  
  • Yeager, C. M., Bottomley, P. J., Arp, D. J. (2001). Cytotoxicity Associated with Trichloroethylene Oxidation in Burkholderia cepacia G4. Appl. Environ. Microbiol. 67: 2107-2115 [Abstract] [Full Text]  
  • McClay, K., Fox, B. G., Steffan, R. J. (2000). Toluene Monooxygenase-Catalyzed Epoxidation of Alkenes. Appl. Environ. Microbiol. 66: 1877-1882 [Abstract] [Full Text]  
  • van Hylckama Vlieg, J. E. T., Kingma, J., Kruizinga, W., Janssen, D. B. (1999). Purification of a Glutathione S-Transferase and a Glutathione Conjugate-Specific Dehydrogenase Involved in Isoprene Metabolism in Rhodococcus sp. Strain AD45. J. Bacteriol. 181: 2094-2101 [Abstract] [Full Text]  
  • Chu, K.-H., Alvarez-Cohen, L. (1999). Evaluation of Toxic Effects of Aeration and Trichloroethylene Oxidation on Methanotrophic Bacteria Grown with Different Nitrogen Sources. Appl. Environ. Microbiol. 65: 766-772 [Abstract] [Full Text]  
  • van Hylckama Vlieg, J. E. T., Kingma, J., van den Wijngaard, A. J., Janssen, D. B. (1998). A Glutathione S-Transferase with Activity towards cis-1,2-Dichloroepoxyethane Is Involved in Isoprene Utilization by Rhodococcus sp. Strain AD45. Appl. Environ. Microbiol. 64: 2800-2805 [Abstract] [Full Text]  
  • Gisi, D., Willi, L., Traber, H., Leisinger, T., Vuilleumier, S. (1998). Effects of Bacterial Host and Dichloromethane Dehalogenase on the Competitiveness of Methylotrophic Bacteria Growing with Dichloromethane. Appl. Environ. Microbiol. 64: 1194-1202 [Abstract] [Full Text]  
  • Mars, A. E., Prins, G. T., Wietzes, P., Koning, W. d., Janssen, D. B. (1998). Effect of Trichloroethylene on the Competitive Behavior of Toluene-Degrading Bacteria. Appl. Environ. Microbiol. 64: 208-215 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»