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Appl. Environ. Microbiol., 09 1996, 3462-3465, Vol 62, No. 9
Copyright © 1996, American Society for Microbiology

Typing of bovine attaching and effacing Escherichia coli by multiplex in vitro amplification of virulence-associated genes

B China, V Pirson and J Mainil
Laboratory of Bacteriology, Faculty of Veterinary Medicine, University of Liege, Belgium.

Attaching and effacing Escherichia coli is a new causal agent of diarrhea in calves. Its major virulence factors are the intimin protein, encoded by the eaeA gene, and the Shiga-like toxins, encoded by slt genes. Because the sequences of these genes are available, we selected specific primers to amplify each virulence gene so as to develop a new identification test based on multiplex amplification of virulence-associated genes. Of 30 tested strains, 14 were eaeA+, 15 were eaeA+ slt-I+, 1 was eaeA+ slt-I+ slt-II+, and 1 was eaeA+ slt-II+. The method proved in our hands to be fast and specific and in perfect correlation with the hybridization method.


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