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Appl. Environ. Microbiol., Sep 1996, 3466-3469, Vol 62, No. 9
SB Grant, CP Pendroy, CL Mayer, JK Bellin and CJ Palmer
Municipal sewage was screened for DNA encoding Shiga-like Toxin (SLT) II, a
key protein involved in the virulence of enterohemorrhagic Escherichia
coli. PCR analysis of sewage concentrates showed that DNA encoding SLT II
was present in a single sample of untreated sewage and absent in all other
samples tested (n = 6). Thermotolerant E. coli cultured from the sewage (n
= 1,520) also tested negative for SLT II by colony hybridization.
Copyright © 1996, American Society for Microbiology
Prevalence of enterohemorrhagic Escherichia coli in raw and treated municipal sewage
Department of Civil and Environmental Engineering, University of California, Irvine 92717, USA. sbgrant@uci.edu
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