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Appl. Environ. Microbiol., 09 1996, 3486-3488, Vol 62, No. 9
Copyright © 1996, American Society for Microbiology

Use of green fluorescent protein to monitor survival of genetically engineered bacteria in aquatic environments

LG Leff and AA Leff
Department of Biological Sciences, Kent State University, Ohio 44242, USA. LLEFF@KENTVM.KENT.EDU

Many methods for detecting model genetically engineered microorganisms (GEMs) in experimental ecosystems rely on cultivation of introduced cells. In this study, survival of Escherichia coli was monitored with the green fluorescent protein (GFP) gene. This approach allowed enumeration of GEMs by both plating and microscopy. Use of the GFP- marked GEMs revealed that E. coli persisted in stream water at higher densities as determined microscopically than as determined by CFU enumeration. The GFP gene did not negatively impact the fitness of the host strain.


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