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Appl. Environ. Microbiol., 09 1996, 3494-3498, Vol 62, No. 9
Copyright © 1996, American Society for Microbiology

Cloning and analysis of the restriction-modification system LlaBI, a bacteriophage resistance system from Lactococcus lactis subsp. cremoris W56

NR Nyengaard, J Falkenberg-Klok and J Josephsen
Department of Dairy and Food Science, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.

The genes coding for the type II restriction-modification (R/M) system LlaBI, which recognized the sequence 5'-C decreases TRYAG-3', have been cloned from a plasmid in Lactococcus lactis subsp. cremoris W56 and sequenced. The DNA sequence predicts an endonuclease of 299 amino acids (33 kDa) and a methylase of 580 amino acids (65 kDa). A 4.0-kb HindIII fragment in pSA3 was able to restrict bacteriophages, showing that the cloned R/M system can function as a phage defense mechanism in L. lactis.


This article has been cited by other articles:

  • Su, P., Im, H., Hsieh, H., Kang'A, S., Dunn, N. W. (1999). LlaFI, a Type III Restriction and Modification System in Lactococcus lactis. Appl. Environ. Microbiol. 65: 686-693 [Abstract] [Full Text]  
  • Twomey, D. P., McKay, L. L., O'Sullivan, D. J. (1998). Molecular Characterization of the Lactococcus lactis LlaKR2I Restriction-Modification System and Effect of an IS982 Element Positioned between the Restriction and Modification Genes. J. Bacteriol. 180: 5844-5854 [Abstract] [Full Text]  
  • Madsen, A., Josephsen, J. (1998). Cloning and Characterization of the Lactococcal Plasmid-Encoded Type II Restriction/Modification System, LlaDII. Appl. Environ. Microbiol. 64: 2424-2431 [Abstract] [Full Text]  


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