This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Park, J. I.
Right arrow Articles by Dawes, I. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Park, J. I.
Right arrow Articles by Dawes, I. W.
Agricola
Right arrow Articles by Park, J. I.
Right arrow Articles by Dawes, I. W.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., 10 1997, 3818-3824, Vol 63, No. 10
Copyright © 1997, American Society for Microbiology

The freeze-thaw stress response of the yeast Saccharomyces cerevisiae is growth phase specific and is controlled by nutritional state via the RAS-cyclic AMP signal transduction pathway

JI Park, CM Grant, PV Attfield and IW Dawes
School of Biochemistry and Molecular Genetics, University of New South Wales, Sydney, Australia.

The ability of cells to survive freezing and thawing is expected to depend on the physiological conditions experienced prior to freezing. We examined factors affecting yeast cell survival during freeze-thaw stress, including those associated with growth phase, requirement for mitochondrial functions, and prior stress treatment(s), and the role played by relevant signal transduction pathways. The yeast Saccharomyces cerevisiae was frozen at -20 degrees C for 2 h (cooling rate, less than 4 degrees C min-1) and thawed on ice for 40 min. Supercooling occurred without reducing cell survival and was followed by freezing. Loss of viability was proportional to the freezing duration, indicating that freezing is the main determinant of freeze- thaw damage. Regardless of the carbon source used, the wild-type strain and an isogenic petite mutant ([rho 0]) showed the same pattern of freeze-thaw tolerance throughout growth, i.e., high resistance during lag phase and low resistance during log phase, indicating that the response to freeze-thaw stress is growth phase specific and not controlled by glucose repression. In addition, respiratory ability and functional mitochondria are necessary to confer full resistance to freeze-thaw stress. Both nitrogen and carbon source starvation led to freeze-thaw tolerance. The use of strains affected in the RAS-cyclic AMP (RAS-cAMP) pathway or supplementation of an rca1 mutant (defective in the cAMP phosphodiesterase gene) with cAMP showed that the freeze- thaw response of yeast is under the control of the RAS-cAMP pathway. Yeast did not adapt to freeze-thaw stress following repeated freeze- thaw treatment with or without a recovery period between freeze-thaw cycles, nor could it adapt following pretreatment by cold shock. However, freeze-thaw tolerance of yeast cells was induced during fermentative and respiratory growth by pretreatment with H2O2, cycloheximide, mild heat shock, or NaCl, indicating that cross protection between freeze-thaw stress and a limited number of other types of stress exists.


This article has been cited by other articles:

  • Lu, C., Brauer, M. J., Botstein, D. (2009). Slow Growth Induces Heat-Shock Resistance in Normal and Respiratory-deficient Yeast. Mol. Biol. Cell 20: 891-903 [Abstract] [Full Text]  
  • Lupi, R., Guerra, S. D., Bugliani, M., Boggi, U., Mosca, F., Torri, S., Prato, S. D., Marchetti, P. (2006). The direct effects of the angiotensin-converting enzyme inhibitors, zofenoprilat and enalaprilat, on isolated human pancreatic islets. Eur J Endocrinol 154: 355-361 [Abstract] [Full Text]  
  • Du, X., Takagi, H. (2005). N-Acetyltransferase Mpr1 Confers Freeze Tolerance on Saccharomyces cerevisiae by Reducing Reactive Oxygen Species. J Biochem 138: 391-397 [Abstract] [Full Text]  
  • Valli, M., Sauer, M., Branduardi, P., Borth, N., Porro, D., Mattanovich, D. (2005). Intracellular pH Distribution in Saccharomyces cerevisiae Cell Populations, Analyzed by Flow Cytometry. Appl. Environ. Microbiol. 71: 1515-1521 [Abstract] [Full Text]  
  • Park, J.-I., Collinson, E. J., Grant, C. M., Dawes, I. W. (2005). Rom2p, the Rho1 GTP/GDP Exchange Factor of Saccharomyces cerevisiae, Can Mediate Stress Responses via the Ras-cAMP Pathway. J. Biol. Chem. 280: 2529-2535 [Abstract] [Full Text]  
  • Tanghe, A., Van Dijck, P., Colavizza, D., Thevelein, J. M. (2004). Aquaporin-Mediated Improvement of Freeze Tolerance of Saccharomyces cerevisiae Is Restricted to Rapid Freezing Conditions. Appl. Environ. Microbiol. 70: 3377-3382 [Abstract] [Full Text]  
  • Terao, Y., Nakamori, S., Takagi, H. (2003). Gene Dosage Effect of L-Proline Biosynthetic Enzymes on L-Proline Accumulation and Freeze Tolerance in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 69: 6527-6532 [Abstract] [Full Text]  
  • Tanghe, A., Van Dijck, P., Dumortier, F., Teunissen, A., Hohmann, S., Thevelein, J. M. (2002). Aquaporin Expression Correlates with Freeze Tolerance in Baker's Yeast, and Overexpression Improves Freeze Tolerance in Industrial Strains. Appl. Environ. Microbiol. 68: 5981-5989 [Abstract] [Full Text]  
  • Teunissen, A., Dumortier, F., Gorwa, M.-F., Bauer, J., Tanghe, A., Loiez, A., Smet, P., Van Dijck, P., Thevelein, J. M. (2002). Isolation and Characterization of a Freeze-Tolerant Diploid Derivative of an Industrial Baker's Yeast Strain and Its Use in Frozen Doughs. Appl. Environ. Microbiol. 68: 4780-4787 [Abstract] [Full Text]  
  • Hohmann, S. (2002). Osmotic Stress Signaling and Osmoadaptation in Yeasts. Microbiol. Mol. Biol. Rev. 66: 300-372 [Abstract] [Full Text]  
  • Causton, H. C., Ren, B., Koh, S. S., Harbison, C. T., Kanin, E., Jennings, E. G., Lee, T. I., True, H. L., Lander, E. S., Young, R. A. (2001). Remodeling of Yeast Genome Expression in Response to Environmental Changes. Mol. Biol. Cell 12: 323-337 [Abstract] [Full Text]  
  • Soto, T., Fernández, J., Vicente-Soler, J., Cansado, J., Gacto, M. (1999). Accumulation of Trehalose by Overexpression of tps1, Coding for Trehalose-6-Phosphate Synthase, Causes Increased Resistance to Multiple Stresses in the Fission Yeast Schizosaccharomyces pombe. Appl. Environ. Microbiol. 65: 2020-2024 [Abstract] [Full Text]  
  • Park, J.-I., Grant, C. M., Davies, M. J., Dawes, I. W. (1998). The Cytoplasmic Cu,Zn Superoxide Dismutase of Saccharomyces cerevisiae Is Required for Resistance to Freeze-Thaw Stress. GENERATION OF FREE RADICALS DURING FREEZING AND THAWING. J. Biol. Chem. 273: 22921-22928 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»