This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Faude, U. C. Articles by Hofle, M. G. Search for Related Content PubMed PubMed Citation Articles by Faude, U. C. Articles by Hofle, M. G. Agricola Articles by Faude, U. C. Articles by Hofle, M. G.

 Previous Article  |  Next Article 

Appl. Environ. Microbiol., 11 1997, 4534-4542, Vol 63, No. 11
Copyright © 1997, American Society for Microbiology

Development and application of monoclonal antibodies for in situ detection of indigenous bacterial strains in aquatic ecosystems

UC Faude and MG Hofle
Gesellschaft fur Biotechnologische Forschung mbH, AG Mikrobielle Okologie, Braunschweig, Germany.

Strain-specific monoclonal antibodies (MAbs) were developed for three different bacterial isolates obtained from a freshwater environment (Lake Plusssee) in the spring of 1990. The three isolates, which were identified by molecular methods, were as follows: Cytophaga johnsonae PX62, Comamonas acidovorans PX54, and Aeromonas hydrophila PU7718. These strains represented three species that were detected in high abundance during a set of mesocosm experiments in Lake Plusssee by the direct analysis of low-molecular-weight RNAs from bacterioplankton. We developed one MAb each for the bacterial isolates PX54 and PU7718 that did not show any cross-reactivity with other bacterial strains by immunofluorescence microscopy. Each MAb recognized the general lipopolysaccharide fraction of the homologous strain. These MAbs were tested successfully for their ability to be used for the in situ detection and counting of bacteria in lake water by immunofluorescence microscopy. During the spring of 1993, A. hydrophila PU7718 showed a depth distribution in Lake Plusssee with a pronounced maximum abundance at 6 m, whereas Comamonas acidovorans PX54 showed a depth distribution with a maximum abundance at the surface. The application of these MAbs to the freshwater samples enabled us to determine the cell morphologies and microhabitats of these strains within their natural environment. The presence of as many as 8,000 cells of these strains per ml in their original habitats 3 years after their initial isolation demonstrated the persistence of individual strains of heterotrophic bacteria over long time spans in pelagic habitats.

This article has been cited by other articles:

• Dominik, K., Hofle, M. G. (2002). Changes in Bacterioplankton Community Structure and Activity with Depth in a Eutrophic Lake as Revealed by 5S rRNA Analysis. Appl. Environ. Microbiol. 68: 3606-3613 [Abstract] [Full Text]  
• Pougnard, C., Catala, P., Drocourt, J.-L., Legastelois, S., Pernin, P., Pringuez, E., Lebaron, P. (2002). Rapid Detection and Enumeration of Naegleria fowleri in Surface Waters by Solid-Phase Cytometry. Appl. Environ. Microbiol. 68: 3102-3107 [Abstract] [Full Text]  
• Pernthaler, A., Preston, C. M., Pernthaler, J., DeLong, E. F., Amann, R. (2002). Comparison of Fluorescently Labeled Oligonucleotide and Polynucleotide Probes for the Detection of Pelagic Marine Bacteria and Archaea. Appl. Environ. Microbiol. 68: 661-667 [Abstract] [Full Text]  
• Hahn, M. W., Höfle, M. G. (1999). Flagellate Predation on a Bacterial Model Community: Interplay of Size-Selective Grazing, Specific Bacterial Cell Size, and Bacterial Community Composition. Appl. Environ. Microbiol. 65: 4863-4872 [Abstract] [Full Text]  
• Höfle, M. G., Haas, H., Dominik, K. (1999). Seasonal Dynamics of Bacterioplankton Community Structure in a Eutrophic Lake as Determined by 5S rRNA Analysis. Appl. Environ. Microbiol. 65: 3164-3174 [Abstract] [Full Text]  
• Lee, A., Chow, D., Haus, B., Tseng, W., Evans, D., Fleiszig, S., Chandy, G., Machen, T. (1999). Airway epithelial tight junctions and binding and cytotoxicity of Pseudomonas aeruginosa. Am. J. Physiol. Lung Cell. Mol. Physiol. 277: L204-L217 [Abstract] [Full Text]  
• Hahn, M. W., Moore, E. R. B., Höfle, M. G. (1999). Bacterial Filament Formation, a Defense Mechanism against Flagellate Grazing, Is Growth Rate Controlled in Bacteria of Different Phyla. Appl. Environ. Microbiol. 65: 25-35 [Abstract] [Full Text]  
• Pernthaler, J., Glöckner, F.-O., Unterholzner, S., Alfreider, A., Psenner, R., Amann, R. (1998). Seasonal Community and Population Dynamics of Pelagic Bacteria and Archaea in a High Mountain Lake. Appl. Environ. Microbiol. 64: 4299-4306 [Abstract] [Full Text]  
• Weinbauer, M. G., Höfle, M. G. (1998). Distribution and Life Strategies of Two Bacterial Populations in a Eutrophic Lake. Appl. Environ. Microbiol. 64: 3776-3783 [Abstract] [Full Text]  
• Hahn, M. W., Höfle, M. G. (1998). Grazing Pressure by a Bacterivorous Flagellate Reverses the Relative Abundance of Comamonas acidovorans PX54 and Vibrio Strain CB5 in Chemostat Cocultures. Appl. Environ. Microbiol. 64: 1910-1918 [Abstract] [Full Text]