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Appl. Environ. Microbiol., Nov 1997, 4597-4599, Vol 63, No. 11
Copyright © 1997, American Society for Microbiology

DNase Activities of the Extracellular, Cell Wall-Associated, and Cytoplasmic Protein Fractions of Frankia Strain R43

F Tavares and A Sellstedt
Institute of Botany and Institute for Molecular and Cell Biology, University of Porto, 4150 Porto, Portugal and Department of Plant Physiology, UmeA University, S-901 87 UmeA, Sweden

DNase activities in different protein fractions of Frankia strain R43 were studied. The extracellular and the cell wall-associated fractions revealed the presence of exo- and endonucleolytic enzymes, but none was detected in the cytoplasmic fraction. The strongest DNase hydrolysis was found in the extracellular fraction, in which six DNases were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.